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Journal Article

Regulation of Na+-Pi cotransport by 1,25-dihydroxyvitamin D3 in rabbit duodenal brush-border membrane

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Hildmann,  Bruno
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Murer,  Heini
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Hildmann, B., Storelli, C., Danisi, G., & Murer, H. (1982). Regulation of Na+-Pi cotransport by 1,25-dihydroxyvitamin D3 in rabbit duodenal brush-border membrane. American Journal of Physiology-Gastrointestinal and Liver Physiology, 242(5), G533-G539. doi:10.1152/ajpgi.1982.242.5.G533.


Cite as: https://hdl.handle.net/21.11116/0000-0008-1CE2-A
Abstract
Brush-border membrane vesicles were isolated from rabbit duodenum by a Mg2+ precipitation method, and phosphate transport was analyzed by a rapid filtration technique. Uptake of inorganic phosphate (Pi) was stimulated by an inwardly directed sodium gradient, indicating the operation of a Na-Pi cotransport system in brush-border membrane vesicles. Treatment of the animals with ethane-1-hydroxy-1,1-diphosphonate (EHDP), which is known to decrease the circulating levels of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], reduced within 3 days the sodium-dependent Pi transport in the brush-border vesicles. Injections of 1,25(OH)2D3 into rabbits increased within 9 h the sodium-dependent Pi transport in membranes from EHDP-treated animals as well as in untreated ones. The Na-D-glucose cotransport system appeared to be unaffected by these maneuvers. These results suggest that the Na-Pi cotransport system is an important site of regulation of intestinal transepithelial Pi transport by 1,25(OH)2)D3.