Asymmetric GUVs Prepared by M beta CD-Mediated Lipid Exchange: An FCS Study

Chiantia, Salvatore; Schwille, Petra; Klymchenko, Andrey S.; London, Erwin

doi:10.1016/j.bpj.2010.11.051

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Asymmetric GUVs Prepared by M beta CD-Mediated Lipid Exchange: An FCS Study

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1-s2.0-S0006349510014785-mmc1.pdf
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Citation

Chiantia, S., Schwille, P., Klymchenko, A. S., & London, E. (2011). Asymmetric GUVs Prepared by M beta CD-Mediated Lipid Exchange: An FCS Study. Biophysical Journal, 100(1), L1-L3. doi:10.1016/j.bpj.2010.11.051.

Cite as: https://hdl.handle.net/21.11116/0000-0008-4B65-3

Abstract

We report a simple method to obtain stable asymmetric giant unilamellar vesicles (GUVs). Fluorescence correlation spectroscopy was used to quantitatively characterize vesicle properties. After brain sphingomyelin (bSM) was exchanged into dioleoylphosphatidylcholine (DOPC) GUVs, lateral diffusion in the bSM-containing outer leaflet decreased, whereas that in the DOPC-containing inner leaflet was largely unchanged, confirming asymmetry and a lack of coupling between the physical states of the inner and outer leaflets. In contrast, after bSM was exchanged into brain phosphatidylcholine vesicles, lateral diffusion decreased in both leaflets. Thus, asymmetric GUVs should be useful for investigating the molecular mechanisms behind interleaflet coupling.