English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse
  1. Basket / 
  2. Item Summary

Item

ITEM ACTIONSEXPORT
Add to Basket
  DetailsSummary
 PreviousNext  

Released
Journal Article

The Desensitized Channelrhodopsin-2 Photointermediate Contains 13-cis,15-syn Retinal Schiff Base

MPS-Authors
/persons/resource/persons137591

Bamann,  Christian
Emeritusgruppe Biophysikalische Chemie, Max Planck Institute of Biophysics, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Becker-Baldus, J., Leeder, A., Brown, L. J., Brown, R. C. D., Bamann, C., & Glaubitz, C. (2021). The Desensitized Channelrhodopsin-2 Photointermediate Contains 13-cis,15-syn Retinal Schiff Base. Angewandte Chemie, International Edition in English, 60. doi:10.1002/anie.202015797.


Cite as: https://hdl.handle.net/21.11116/0000-0008-85A1-B
Abstract
Channelrhodopsin-2 (ChR2) is a light-gated cation channel and was used to lay the foundations of optogenetics. Its dark state X-ray structure has been determined in 2017 for the wild-type, which is the prototype for all other ChR variants. However, the mechanistic understanding of the channel function is still incomplete in terms of structural changes after photon absorption by the retinal chromophore and in the framework of functional models. Hence, detailed information needs to be collected on the dark state as well as on the different photointermediates. For ChR-2 detailed knowledge on the chromophore configuration in the different states is still missing and a consensus has not been achieved. Using DNP-enhanced solid-state MAS NMR spectroscopy on proteoliposome samples, we unambiguously determine the chromophore configuration in the desensitized state, and we show that this state occurs towards the end of the photocycle.