Structure of RNA polymerase II pre-initiation complex at 2.9 Å defines initial 
DNA opening

Schilbach, S.; Aibara, S.; Dienemann, C.; Grabbe, F.; Cramer, P.

doi:10.1016/j.cell.2021.05.012

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Structure of RNA polymerase II pre-initiation complex at 2.9 Å defines initial DNA opening

MPS-Authors

/persons/resource/persons211612

Schilbach, S.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons256165

Aibara, S.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons195449

Dienemann, C.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons262761

Grabbe, F.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons127020

Cramer, P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Schilbach, S., Aibara, S., Dienemann, C., Grabbe, F., & Cramer, P. (2021). Structure of RNA polymerase II pre-initiation complex at 2.9 Å defines initial DNA opening. Cell, 184(15), 4064-4072.e28. doi:10.1016/j.cell.2021.05.012.

引用: https://hdl.handle.net/21.11116/0000-0008-C2BF-6

要旨

Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of the yeast PIC and define the mechanism of initial DNA opening. We trap the PIC in an intermediate state that contains half a turn of open DNA located 30–35 base pairs downstream of the TATA box. The initially opened DNA region is flanked and stabilized by the polymerase “clamp head loop” and the TFIIF “charged region” that both contribute to promoter-initiated transcription. TFIIE facilitates initiation by buttressing the clamp head loop and by regulating the TFIIH translocase. The initial DNA bubble is then extended in the upstream direction, leading to the open promoter complex and enabling start-site scanning and RNA synthesis. This unique mechanism of DNA opening may permit more intricate regulation than in the Pol I and Pol III systems.