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Journal Article

Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection.

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Stepien,  Barbara
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Vaid,  Samir
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Naumann,  Ronald
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Huttner,  Wieland
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Stepien, B., Vaid, S., Naumann, R., Holtz, A., & Huttner, W. (2021). Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection. STAR protocols, 2(2): 100494. doi:10.1016/j.xpro.2021.100494.


Cite as: https://hdl.handle.net/21.11116/0000-0008-DAB3-8
Abstract
Interspecies chimerism is a useful tool to study interactions between cells of different genetic makeup in order to elucidate the mechanisms underlying non-cell-autonomous processes, including evolutionary events. However, generating interspecies chimeras with high efficiency and chimerism level remains challenging. Here, we describe a protocol for generating chimeras between mouse and rat. Donor embryonic stem cells of one species are microinjected into early embryos of the other species (recipient), which are implanted into host foster mothers of the recipient species. For complete details on the use and execution of this protocol, please refer to Stepien et al. (2020).