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Journal Article

DNA Segregation in Natural and Synthetic Minimal Systems

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Hürtgen,  Daniel
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Murray,  Sean M.
Research Group Mechanisms of Spatial-Organisation, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Mascarenhas,  Judita
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Sourjik,  Victor
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Hürtgen, D., Murray, S. M., Mascarenhas, J., & Sourjik, V. (2019). DNA Segregation in Natural and Synthetic Minimal Systems. Advanced Biosystems, 3(6): 1800316. doi:10.1002/adbi.201800316.


Cite as: https://hdl.handle.net/21.11116/0000-0008-ECAF-A
Abstract
Faithful segregation of replicated genomes to dividing daughter cells is a major hallmark of cellular life and needs to be part of the future design of the robustly proliferating minimal cell. So far, the complexity of eukaryotic chromosome segregation machineries has limited their applicability to synthetic systems. Prokaryotic plasmid segregation machineries offer promising alternative tools for bottom-up synthetic biology, with the first three-component DNA segregation system being reconstituted a decade ago. In this review, the mechanisms underlying DNA segregation in prokaryotes, with a particular focus on segregation of plasmids and chromosomal replication origins are reviewed, along with a brief discussion of archaeal and eukaryotic systems. In addition, this review shows how in vitro reconstitution has allowed deeper insights into these processes and discusses possible applications of these machineries for a minimal synthetic segrosome as well as the challenge of its coupling to a minimal replisome.