Item

ITEM ACTIONS

This item is discarded!DetailsSummary

Discarded

Book Chapter

Glycoproteomics Technologies in Glycobiotechnology

MPS-Authors

/persons/resource/persons121091

Alagesan, Kathirvel
Max Planck Unit for the Science of Pathogens, Max Planck Society;

/persons/resource/persons86327

Hoffmann, Marcus
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

/persons/resource/persons86442

Rapp, Erdmann
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;
Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

External Resource

(No access)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Alagesan, K., Hoffmann, M., Rapp, E., & Kolarich, D. (2020). Glycoproteomics Technologies in Glycobiotechnology. In Advances in Biochemical Engineering/Biotechnology. Springer. doi:10.1007/10_2020_144.

Abstract

Glycosylation is a key factor determining the pharmacological properties of biotherapeutics, including their stability, solubility, bioavailability, pharmacokinetics, and immunogenicity. As such, comprehensive information about glycosylation of biotherapeutics is critical to demonstrate similarity. Regulatory agencies also require extensive documentation of the comprehensive analyses of glycosylation-related critical quality attributes (CQAs) during the development, manufacturing, and release of biosimilars. Mass spectrometry has catalysed tremendous advancements in the characterisation of glycosylation CQAs of biotherapeutics. Here we provide a perspective overview on the MS-based technologies relevant for biotherapeutic product characterisation with an emphasis on the recent developments that allow determination of glycosylation features such as site of glycosylation, sialic acid linkage, glycan structure, and content.Graphical AbstractOpen image in new window