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Markus Krämer
Ann-Kathrin Kissmann
Heinz Fabian Raber
Hu Xing
Patrizia Favella
Ingrid Müller
Barbara Spellerberg
Tanja Weil
Dennis Kubiczek
Susanne Sihler
Ulrich Ziener and Frank Rosenau
aptamers
hydrogel beads
Pseudomonas aeruginosa
sepsis
Systemic blood stream infections are a major threat to human health and are dramatically increasing worldwide. Pseudomonas aeruginosa is a WHO-alerted multi-resistant pathogen of extreme importance as a cause of sepsis. Septicemia patients have significantly increased survival chances if sepsis is diagnosed in the early stages. Affinity materials can not only represent attractive tools for specific diagnostics of pathogens in the blood but can prospectively also serve as the technical foundation of therapeutic filtration devices. Based on the recently developed aptamers directed against P. aeruginosa, we here present aptamer-functionalized beads for specific binding of this pathogen in blood samples. These aptamer capture beads (ACBs) are manufactured by crosslinking bovine serum albumin (BSA) in an emulsion and subsequent functionalization with the amino-modified aptamers on the bead surface using the thiol- and amino-reactive bispecific crosslinker PEG4-SPDP. Specific and quantitative binding of P. aeruginosa as the dedicated target of the ACBs was demonstrated in serum and blood. These initial but promising results may open new routes for the development of ACBs as a platform technology for fast and reliable diagnosis of bloodstream infections and, in the long term, blood filtration techniques in the fight against sepsis.
BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for Capturing Pseudomonas aeruginosa in Serum and Blood
application/pdf
2021-10-15T12:21:02+02:00
LaTeX with hyperref
2021-10-15T13:09:18+02:00
aptamers; hydrogel beads; Pseudomonas aeruginosa; sepsis
pdfTeX-1.40.21
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