date: 2021-10-27T08:20:27Z
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pdf:docinfo:title: Albumin Microspheres as ?Trans-Ferry-Beads? for Easy Cell Passaging in Cell Culture Technology
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subject: Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N?-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles. They allow a significant simplification of an indispensable and laborious step in traditional cell culture workflows. This cell passaging (or splitting) to fresh culture vessels/flasks conventionally requires harsh trypsinization, which can be omitted by using the ?trans-ferry-beads? presented here. When added to different pre-cultivated adherent cell lines, the beads are efficiently boarded by cells as passengers and can be easily transferred afterward for the embarkment of novel flasks. After this procedure, cells are perfectly viable and show normal growth behavior. Thus, the trans-ferry-beads not only may become extremely affordable as a final product but also may generally replace trypsinization in conventional cell culture, thereby opening new routes for the establishment of optimized and resource-efficient workflows in biological and medical cell culture laboratories.
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dc:title: Albumin Microspheres as ?Trans-Ferry-Beads? for Easy Cell Passaging in Cell Culture Technology
modified: 2021-10-27T08:20:27Z
cp:subject: Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N?-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles. They allow a significant simplification of an indispensable and laborious step in traditional cell culture workflows. This cell passaging (or splitting) to fresh culture vessels/flasks conventionally requires harsh trypsinization, which can be omitted by using the ?trans-ferry-beads? presented here. When added to different pre-cultivated adherent cell lines, the beads are efficiently boarded by cells as passengers and can be easily transferred afterward for the embarkment of novel flasks. After this procedure, cells are perfectly viable and show normal growth behavior. Thus, the trans-ferry-beads not only may become extremely affordable as a final product but also may generally replace trypsinization in conventional cell culture, thereby opening new routes for the establishment of optimized and resource-efficient workflows in biological and medical cell culture laboratories.
pdf:docinfo:subject: Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N?-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles. They allow a significant simplification of an indispensable and laborious step in traditional cell culture workflows. This cell passaging (or splitting) to fresh culture vessels/flasks conventionally requires harsh trypsinization, which can be omitted by using the ?trans-ferry-beads? presented here. When added to different pre-cultivated adherent cell lines, the beads are efficiently boarded by cells as passengers and can be easily transferred afterward for the embarkment of novel flasks. After this procedure, cells are perfectly viable and show normal growth behavior. Thus, the trans-ferry-beads not only may become extremely affordable as a final product but also may generally replace trypsinization in conventional cell culture, thereby opening new routes for the establishment of optimized and resource-efficient workflows in biological and medical cell culture laboratories.
pdf:docinfo:creator: Patrizia Favella, Susanne Sihler, Heinz Raber, Ann-Kathrin Kissmann, Markus Krämer, Valerie Amann, Dennis Kubiczek, Jennifer Baatz, Fabian Lang, Fabian Port, Kay-Eberhard Gottschalk, Daniel Mayer, Barbara Spellerberg, Steffen Stenger, Ingrid Müller, Tanja Weil, Ulrich Ziener and Frank Rosenau
meta:author: Patrizia Favella, Susanne Sihler, Heinz Raber, Ann-Kathrin Kissmann, Markus Krämer, Valerie Amann, Dennis Kubiczek, Jennifer Baatz, Fabian Lang, Fabian Port, Kay-Eberhard Gottschalk, Daniel Mayer, Barbara Spellerberg, Steffen Stenger, Ingrid Müller, Tanja Weil, Ulrich Ziener and Frank Rosenau
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created: 2021-10-27T06:21:24Z
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Author: Patrizia Favella, Susanne Sihler, Heinz Raber, Ann-Kathrin Kissmann, Markus Krämer, Valerie Amann, Dennis Kubiczek, Jennifer Baatz, Fabian Lang, Fabian Port, Kay-Eberhard Gottschalk, Daniel Mayer, Barbara Spellerberg, Steffen Stenger, Ingrid Müller, Tanja Weil, Ulrich Ziener and Frank Rosenau
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dc:description: Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N?-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles. They allow a significant simplification of an indispensable and laborious step in traditional cell culture workflows. This cell passaging (or splitting) to fresh culture vessels/flasks conventionally requires harsh trypsinization, which can be omitted by using the ?trans-ferry-beads? presented here. When added to different pre-cultivated adherent cell lines, the beads are efficiently boarded by cells as passengers and can be easily transferred afterward for the embarkment of novel flasks. After this procedure, cells are perfectly viable and show normal growth behavior. Thus, the trans-ferry-beads not only may become extremely affordable as a final product but also may generally replace trypsinization in conventional cell culture, thereby opening new routes for the establishment of optimized and resource-efficient workflows in biological and medical cell culture laboratories.
Keywords: hydrogel; beads; cell culture; cell transfer
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dc:creator: Patrizia Favella, Susanne Sihler, Heinz Raber, Ann-Kathrin Kissmann, Markus Krämer, Valerie Amann, Dennis Kubiczek, Jennifer Baatz, Fabian Lang, Fabian Port, Kay-Eberhard Gottschalk, Daniel Mayer, Barbara Spellerberg, Steffen Stenger, Ingrid Müller, Tanja Weil, Ulrich Ziener and Frank Rosenau
description: Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N?-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles. They allow a significant simplification of an indispensable and laborious step in traditional cell culture workflows. This cell passaging (or splitting) to fresh culture vessels/flasks conventionally requires harsh trypsinization, which can be omitted by using the ?trans-ferry-beads? presented here. When added to different pre-cultivated adherent cell lines, the beads are efficiently boarded by cells as passengers and can be easily transferred afterward for the embarkment of novel flasks. After this procedure, cells are perfectly viable and show normal growth behavior. Thus, the trans-ferry-beads not only may become extremely affordable as a final product but also may generally replace trypsinization in conventional cell culture, thereby opening new routes for the establishment of optimized and resource-efficient workflows in biological and medical cell culture laboratories.
dcterms:created: 2021-10-27T06:21:24Z
Last-Modified: 2021-10-27T08:20:27Z
dcterms:modified: 2021-10-27T08:20:27Z
title: Albumin Microspheres as ?Trans-Ferry-Beads? for Easy Cell Passaging in Cell Culture Technology
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pdf:docinfo:keywords: hydrogel; beads; cell culture; cell transfer
pdf:docinfo:modified: 2021-10-27T08:20:27Z
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creator: Patrizia Favella, Susanne Sihler, Heinz Raber, Ann-Kathrin Kissmann, Markus Krämer, Valerie Amann, Dennis Kubiczek, Jennifer Baatz, Fabian Lang, Fabian Port, Kay-Eberhard Gottschalk, Daniel Mayer, Barbara Spellerberg, Steffen Stenger, Ingrid Müller, Tanja Weil, Ulrich Ziener and Frank Rosenau
dc:subject: hydrogel; beads; cell culture; cell transfer
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