Nuclear pores dilate and constrict in cellulo

Zimmerli, Christian E.; Allegretti, Matteo; Rantos, Vasileios; Goetz, Sara K.; Obarska-Kosinska, Agnieszka; Zagoriy, Ievgeniia; Halavatyi, Aliaksandr; Hummer, Gerhard; Mahamid, Julia; Kosinski, Jan; Beck, Martin

doi:10.1126/science.abd9776

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Nuclear pores dilate and constrict in cellulo

MPS-Authors

/persons/resource/persons267301

Zimmerli, Christian E.
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, 69120 Heidelberg, Germany;
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany;

/persons/resource/persons137581

Allegretti, Matteo
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany;

/persons/resource/persons244756

Obarska-Kosinska, Agnieszka
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
EMBL Hamburg, Hamburg, Germany;

/persons/resource/persons15259

Hummer, Gerhard
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;
Institute of Biophysics, Goethe University Frankfurt, Frankfurt am Main, Germany;

/persons/resource/persons232071

Beck, Martin
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

science.abd9776.pdf
(全文テキスト（全般）), 4MB

付随資料 (公開)

There is no public supplementary material available

引用

Zimmerli, C. E., Allegretti, M., Rantos, V., Goetz, S. K., Obarska-Kosinska, A., Zagoriy, I., Halavatyi, A., Hummer, G., Mahamid, J., Kosinski, J., & Beck, M. (2021). Nuclear pores dilate and constrict in cellulo. Science, 374(6573):. doi:10.1126/science.abd9776.

引用: https://hdl.handle.net/21.11116/0000-0009-796D-6

要旨

In eukaryotic cells, nuclear pore complexes (NPCs) fuse the inner and outer nuclear membranes and mediate nucleocytoplasmic exchange. They are made of 30 different nucleoporins and form a cylindrical architecture around an aqueous central channel. This architecture is highly dynamic in space and time. Variations in NPC diameter have been reported, but the physiological circumstances and the molecular details remain unknown. Here we combined cryo-electron tomography (cryo-ET) with integrative structural modeling to capture a molecular movie of the respective large-scale conformational changes in cellulo. While NPCs of exponentially growing cells adopted a dilated conformation, they reversibly constricted upon cellular energy depletion or conditions of hypertonic osmotic stress. Our data point to a model where the nuclear envelope membrane tension is linked to the conformation of the NPC.