date: 2021-10-21T06:47:24Z
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pdf:docinfo:title: Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System
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Keywords: porous polyethylene; biomaterial; material science; albumin nanocarriers; tissue engineering; release kinetics; dorsal skinfold chamber; fluorescence microscopy
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subject: Background: Porous polyethylene (PPE) implants are used for the reconstruction of tissue defects but have a risk of rejection in case of insufficient ingrowth into the host tissue. Various growth factors can promote implant ingrowth, yet a long-term gradient is a prerequisite for the mediation of these effects. As modification of the implant surface with nanocarriers may facilitate a long-term gradient by sustained factor release, implants modified with crosslinked albumin nanocarriers were evaluated in vivo. Methods: Nanocarriers from murine serum albumin (MSA) were prepared by an inverse miniemulsion technique encapsulating either a low- or high-molar mass fluorescent cargo. PPE implants were subsequently coated with these nanocarriers. In control cohorts, the implant was coated with the homologue non-encapsulated cargo substance by dip coating. Implants were consequently analyzed in vivo using repetitive fluorescence microscopy utilizing the dorsal skinfold chamber in mice for ten days post implantation. Results: Implant-modification with MSA nanocarriers significantly prolonged the presence of the encapsulated small molecules while macromolecules were detectable during the investigated timeframe regardless of the form of application. Conclusions: Surface modification of PPE implants with MSA nanocarriers results in the alternation of release kinetics especially when small molecular substances are used and therefore allows a prolonged factor release for the promotion of implant integration.
dc:creator: Jonas Eckrich, Niklas Hoormann, Erik Kersten, Keti Piradashvili, Frederik R. Wurm, Martin Heller, Sven Becker, Toni Anusic, Juergen Brieger and Sebastian Strieth
dcterms:created: 2021-10-21T06:42:45Z
Last-Modified: 2021-10-21T06:47:24Z
dcterms:modified: 2021-10-21T06:47:24Z
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title: Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System
Last-Save-Date: 2021-10-21T06:47:24Z
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pdf:docinfo:keywords: porous polyethylene; biomaterial; material science; albumin nanocarriers; tissue engineering; release kinetics; dorsal skinfold chamber; fluorescence microscopy
pdf:docinfo:modified: 2021-10-21T06:47:24Z
meta:save-date: 2021-10-21T06:47:24Z
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dc:title: Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System
modified: 2021-10-21T06:47:24Z
cp:subject: Background: Porous polyethylene (PPE) implants are used for the reconstruction of tissue defects but have a risk of rejection in case of insufficient ingrowth into the host tissue. Various growth factors can promote implant ingrowth, yet a long-term gradient is a prerequisite for the mediation of these effects. As modification of the implant surface with nanocarriers may facilitate a long-term gradient by sustained factor release, implants modified with crosslinked albumin nanocarriers were evaluated in vivo. Methods: Nanocarriers from murine serum albumin (MSA) were prepared by an inverse miniemulsion technique encapsulating either a low- or high-molar mass fluorescent cargo. PPE implants were subsequently coated with these nanocarriers. In control cohorts, the implant was coated with the homologue non-encapsulated cargo substance by dip coating. Implants were consequently analyzed in vivo using repetitive fluorescence microscopy utilizing the dorsal skinfold chamber in mice for ten days post implantation. Results: Implant-modification with MSA nanocarriers significantly prolonged the presence of the encapsulated small molecules while macromolecules were detectable during the investigated timeframe regardless of the form of application. Conclusions: Surface modification of PPE implants with MSA nanocarriers results in the alternation of release kinetics especially when small molecular substances are used and therefore allows a prolonged factor release for the promotion of implant integration.
pdf:docinfo:subject: Background: Porous polyethylene (PPE) implants are used for the reconstruction of tissue defects but have a risk of rejection in case of insufficient ingrowth into the host tissue. Various growth factors can promote implant ingrowth, yet a long-term gradient is a prerequisite for the mediation of these effects. As modification of the implant surface with nanocarriers may facilitate a long-term gradient by sustained factor release, implants modified with crosslinked albumin nanocarriers were evaluated in vivo. Methods: Nanocarriers from murine serum albumin (MSA) were prepared by an inverse miniemulsion technique encapsulating either a low- or high-molar mass fluorescent cargo. PPE implants were subsequently coated with these nanocarriers. In control cohorts, the implant was coated with the homologue non-encapsulated cargo substance by dip coating. Implants were consequently analyzed in vivo using repetitive fluorescence microscopy utilizing the dorsal skinfold chamber in mice for ten days post implantation. Results: Implant-modification with MSA nanocarriers significantly prolonged the presence of the encapsulated small molecules while macromolecules were detectable during the investigated timeframe regardless of the form of application. Conclusions: Surface modification of PPE implants with MSA nanocarriers results in the alternation of release kinetics especially when small molecular substances are used and therefore allows a prolonged factor release for the promotion of implant integration.
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pdf:docinfo:creator: Jonas Eckrich, Niklas Hoormann, Erik Kersten, Keti Piradashvili, Frederik R. Wurm, Martin Heller, Sven Becker, Toni Anusic, Juergen Brieger and Sebastian Strieth
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creator: Jonas Eckrich, Niklas Hoormann, Erik Kersten, Keti Piradashvili, Frederik R. Wurm, Martin Heller, Sven Becker, Toni Anusic, Juergen Brieger and Sebastian Strieth
meta:author: Jonas Eckrich, Niklas Hoormann, Erik Kersten, Keti Piradashvili, Frederik R. Wurm, Martin Heller, Sven Becker, Toni Anusic, Juergen Brieger and Sebastian Strieth
dc:subject: porous polyethylene; biomaterial; material science; albumin nanocarriers; tissue engineering; release kinetics; dorsal skinfold chamber; fluorescence microscopy
meta:creation-date: 2021-10-21T06:42:45Z
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meta:keyword: porous polyethylene; biomaterial; material science; albumin nanocarriers; tissue engineering; release kinetics; dorsal skinfold chamber; fluorescence microscopy
Author: Jonas Eckrich, Niklas Hoormann, Erik Kersten, Keti Piradashvili, Frederik R. Wurm, Martin Heller, Sven Becker, Toni Anusic, Juergen Brieger and Sebastian Strieth
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