date: 2022-03-15T10:22:48Z
pdf:PDFVersion: 1.7
pdf:docinfo:title: High-Titer Hepatitis C Virus Production in a Scalable Single-Use High Cell Density Bioreactor
xmp:CreatorTool: LaTeX with hyperref
access_permission:can_print_degraded: true
subject: Hepatitis C virus (HCV) infections pose a major public health burden due to high chronicity rates and associated morbidity and mortality. A vaccine protecting against chronic infection is not available but would be important for global control of HCV infections. In this study, cell culture-based HCV production was established in a packed-bed bioreactor (CelCradle?) aiming to further the development of an inactivated whole virus vaccine and to facilitate virological and immunological studies requiring large quantities of virus particles. HCV was produced in human hepatoma-derived Huh7.5 cells maintained in serum-free medium on days of virus harvesting. Highest virus yields were obtained when the culture was maintained with two medium exchanges per day. However, increasing the total number of cells in the culture vessel negatively impacted infectivity titers. Peak infectivity titers of up to 7.2 log10 focus forming units (FFU)/mL, accumulated virus yields of up to 5.9  1010 FFU, and a cell specific virus yield of up to 41 FFU/cell were obtained from one CelCradle?. CelCradle?-derived and T flask-derived virus had similar characteristics regarding neutralization sensitivity and buoyant density. This packed-bed tide-motion system is available with larger vessels and may thus be a promising platform for large-scale HCV production.
dc:format: application/pdf; version=1.7
pdf:docinfo:creator_tool: LaTeX with hyperref
access_permission:fill_in_form: true
pdf:encrypted: false
dc:title: High-Titer Hepatitis C Virus Production in a Scalable Single-Use High Cell Density Bioreactor
modified: 2022-03-15T10:22:48Z
cp:subject: Hepatitis C virus (HCV) infections pose a major public health burden due to high chronicity rates and associated morbidity and mortality. A vaccine protecting against chronic infection is not available but would be important for global control of HCV infections. In this study, cell culture-based HCV production was established in a packed-bed bioreactor (CelCradle?) aiming to further the development of an inactivated whole virus vaccine and to facilitate virological and immunological studies requiring large quantities of virus particles. HCV was produced in human hepatoma-derived Huh7.5 cells maintained in serum-free medium on days of virus harvesting. Highest virus yields were obtained when the culture was maintained with two medium exchanges per day. However, increasing the total number of cells in the culture vessel negatively impacted infectivity titers. Peak infectivity titers of up to 7.2 log10 focus forming units (FFU)/mL, accumulated virus yields of up to 5.9  1010 FFU, and a cell specific virus yield of up to 41 FFU/cell were obtained from one CelCradle?. CelCradle?-derived and T flask-derived virus had similar characteristics regarding neutralization sensitivity and buoyant density. This packed-bed tide-motion system is available with larger vessels and may thus be a promising platform for large-scale HCV production.
pdf:docinfo:subject: Hepatitis C virus (HCV) infections pose a major public health burden due to high chronicity rates and associated morbidity and mortality. A vaccine protecting against chronic infection is not available but would be important for global control of HCV infections. In this study, cell culture-based HCV production was established in a packed-bed bioreactor (CelCradle?) aiming to further the development of an inactivated whole virus vaccine and to facilitate virological and immunological studies requiring large quantities of virus particles. HCV was produced in human hepatoma-derived Huh7.5 cells maintained in serum-free medium on days of virus harvesting. Highest virus yields were obtained when the culture was maintained with two medium exchanges per day. However, increasing the total number of cells in the culture vessel negatively impacted infectivity titers. Peak infectivity titers of up to 7.2 log10 focus forming units (FFU)/mL, accumulated virus yields of up to 5.9  1010 FFU, and a cell specific virus yield of up to 41 FFU/cell were obtained from one CelCradle?. CelCradle?-derived and T flask-derived virus had similar characteristics regarding neutralization sensitivity and buoyant density. This packed-bed tide-motion system is available with larger vessels and may thus be a promising platform for large-scale HCV production.
pdf:docinfo:creator: Anna Offersgaard, Carlos Rene Duarte Hernandez, Anne Finne Pihl, Nandini Prabhakar Venkatesan, Henrik Krarup, Xiangliang Lin, Udo Reichl, Jens Bukh, Yvonne Genzel and Judith Margarete Gottwein
meta:author: Anna Offersgaard, Carlos Rene Duarte Hernandez, Anne Finne Pihl, Nandini Prabhakar Venkatesan, Henrik Krarup, Xiangliang Lin, Udo Reichl, Jens Bukh, Yvonne Genzel and Judith Margarete Gottwein
meta:creation-date: 2022-02-14T09:09:40Z
created: 2022-02-14T09:09:40Z
access_permission:extract_for_accessibility: true
Creation-Date: 2022-02-14T09:09:40Z
Author: Anna Offersgaard, Carlos Rene Duarte Hernandez, Anne Finne Pihl, Nandini Prabhakar Venkatesan, Henrik Krarup, Xiangliang Lin, Udo Reichl, Jens Bukh, Yvonne Genzel and Judith Margarete Gottwein
producer: pdfTeX-1.40.21
pdf:docinfo:producer: pdfTeX-1.40.21
pdf:unmappedUnicodeCharsPerPage: 17
dc:description: Hepatitis C virus (HCV) infections pose a major public health burden due to high chronicity rates and associated morbidity and mortality. A vaccine protecting against chronic infection is not available but would be important for global control of HCV infections. In this study, cell culture-based HCV production was established in a packed-bed bioreactor (CelCradle?) aiming to further the development of an inactivated whole virus vaccine and to facilitate virological and immunological studies requiring large quantities of virus particles. HCV was produced in human hepatoma-derived Huh7.5 cells maintained in serum-free medium on days of virus harvesting. Highest virus yields were obtained when the culture was maintained with two medium exchanges per day. However, increasing the total number of cells in the culture vessel negatively impacted infectivity titers. Peak infectivity titers of up to 7.2 log10 focus forming units (FFU)/mL, accumulated virus yields of up to 5.9  1010 FFU, and a cell specific virus yield of up to 41 FFU/cell were obtained from one CelCradle?. CelCradle?-derived and T flask-derived virus had similar characteristics regarding neutralization sensitivity and buoyant density. This packed-bed tide-motion system is available with larger vessels and may thus be a promising platform for large-scale HCV production.
Keywords: packed-bed bioreactor; CelCradle?; high cell density cell culture; Huh7.5 cells; whole virus vaccine; inactivated virus vaccine; high-titer HCV production; HCV vaccine development
access_permission:modify_annotations: true
dc:creator: Anna Offersgaard, Carlos Rene Duarte Hernandez, Anne Finne Pihl, Nandini Prabhakar Venkatesan, Henrik Krarup, Xiangliang Lin, Udo Reichl, Jens Bukh, Yvonne Genzel and Judith Margarete Gottwein
description: Hepatitis C virus (HCV) infections pose a major public health burden due to high chronicity rates and associated morbidity and mortality. A vaccine protecting against chronic infection is not available but would be important for global control of HCV infections. In this study, cell culture-based HCV production was established in a packed-bed bioreactor (CelCradle?) aiming to further the development of an inactivated whole virus vaccine and to facilitate virological and immunological studies requiring large quantities of virus particles. HCV was produced in human hepatoma-derived Huh7.5 cells maintained in serum-free medium on days of virus harvesting. Highest virus yields were obtained when the culture was maintained with two medium exchanges per day. However, increasing the total number of cells in the culture vessel negatively impacted infectivity titers. Peak infectivity titers of up to 7.2 log10 focus forming units (FFU)/mL, accumulated virus yields of up to 5.9  1010 FFU, and a cell specific virus yield of up to 41 FFU/cell were obtained from one CelCradle?. CelCradle?-derived and T flask-derived virus had similar characteristics regarding neutralization sensitivity and buoyant density. This packed-bed tide-motion system is available with larger vessels and may thus be a promising platform for large-scale HCV production.
dcterms:created: 2022-02-14T09:09:40Z
Last-Modified: 2022-03-15T10:22:48Z
dcterms:modified: 2022-03-15T10:22:48Z
title: High-Titer Hepatitis C Virus Production in a Scalable Single-Use High Cell Density Bioreactor
xmpMM:DocumentID: uuid:19b0b056-cc12-4473-8638-fa4f8725b34c
Last-Save-Date: 2022-03-15T10:22:48Z
pdf:docinfo:keywords: packed-bed bioreactor; CelCradle?; high cell density cell culture; Huh7.5 cells; whole virus vaccine; inactivated virus vaccine; high-titer HCV production; HCV vaccine development
pdf:docinfo:modified: 2022-03-15T10:22:48Z
meta:save-date: 2022-03-15T10:22:48Z
Content-Type: application/pdf
X-Parsed-By: org.apache.tika.parser.DefaultParser
creator: Anna Offersgaard, Carlos Rene Duarte Hernandez, Anne Finne Pihl, Nandini Prabhakar Venkatesan, Henrik Krarup, Xiangliang Lin, Udo Reichl, Jens Bukh, Yvonne Genzel and Judith Margarete Gottwein
dc:subject: packed-bed bioreactor; CelCradle?; high cell density cell culture; Huh7.5 cells; whole virus vaccine; inactivated virus vaccine; high-titer HCV production; HCV vaccine development
access_permission:assemble_document: true
xmpTPg:NPages: 23
pdf:charsPerPage: 3919
access_permission:extract_content: true
access_permission:can_print: true
meta:keyword: packed-bed bioreactor; CelCradle?; high cell density cell culture; Huh7.5 cells; whole virus vaccine; inactivated virus vaccine; high-titer HCV production; HCV vaccine development
access_permission:can_modify: true
pdf:docinfo:created: 2022-02-14T09:09:40Z