Integration of multiple imaging platforms to uncover cardiovascular defects in 
adult zebrafish

Bensimon-Brito, Anabela; Boezio, Giulia L. M.; Cardeira-da-Silva, Joao; Wietelmann, Astrid; Ramkumar, Srinath; Lundegaard, Pia R.; Helker, Christian S. M.; Ramadass, Radhan; Piesker, Janett; Nauerth, Arno; Mueller, Clemens; Stainier, Didier Y. R.

doi:10.1093/cvr/cvab310

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Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish

MPS-Authors

/persons/resource/persons239395

Bensimon-Brito, Anabela
Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society;

/persons/resource/persons239402

Boezio, Giulia L. M.
Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society;

/persons/resource/persons248802

Cardeira-da-Silva, Joao
Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society;

/persons/resource/persons224397

Wietelmann, Astrid
Small Animal Magnetic Resonance Imaging, Max Planck Institute for Heart and Lung Research, Max Planck Society;

/persons/resource/persons248829

Ramkumar, Srinath
Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society;

/persons/resource/persons224237

Helker, Christian S. M.
Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society;

/persons/resource/persons224268

Ramadass, Radhan
Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society;

/persons/resource/persons248890

Piesker, Janett
Electron Microscopy, Max Planck Institute for Heart and Lung Research, Max Planck Society;

/persons/resource/persons224278

Stainier, Didier Y. R.
Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society;

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引用

Bensimon-Brito, A., Boezio, G. L. M., Cardeira-da-Silva, J., Wietelmann, A., Ramkumar, S., Lundegaard, P. R., Helker, C. S. M., Ramadass, R., Piesker, J., Nauerth, A., Mueller, C., & Stainier, D. Y. R. (2021). Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish. CARDIOVASCULAR RESEARCH,. doi:10.1093/cvr/cvab310.

引用: https://hdl.handle.net/21.11116/0000-000A-779C-1

要旨

Aims Mammalian models have been instrumental in investigating adult heart function and human disease. However, electrophysiological differences with human hearts and high costs motivate the need for non-mammalian models. The zebrafish is a well-established genetic model to study cardiovascular development and function; however, analysis of cardiovascular phenotypes in adult specimens is particularly challenging as they are opaque. Methods and results Here, we optimized and combined multiple imaging techniques including echocardiography, magnetic resonance imaging, and micro-computed tomography to identify and analyse cardiovascular phenotypes in adult zebrafish. Using alk5a/tgfbr1a mutants as a case study, we observed morphological and functional cardiovascular defects that were undetected with conventional approaches. Correlation analysis of multiple parameters revealed an association between haemodynamic defects and structural alterations of the heart, as observed clinically. Conclusion We report a new, comprehensive, and sensitive platform to identify otherwise indiscernible cardiovascular phenotypes in adult zebrafish.