date: 2022-04-07T11:17:20Z
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pdf:docinfo:title: Live Fluorescence Imaging of F-Actin Organization in Chick Whole Embryo Cultures Using SiR-Actin
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subject: Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations.
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dc:title: Live Fluorescence Imaging of F-Actin Organization in Chick Whole Embryo Cultures Using SiR-Actin
modified: 2022-04-07T11:17:20Z
cp:subject: Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations.
pdf:docinfo:subject: Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations.
pdf:docinfo:creator: Manuel Schmitz-Elbers, Gra?vydas Lukinavi?ius and Theodoor H. Smit
meta:author: Manuel Schmitz-Elbers, Gra?vydas Lukinavi?ius and Theodoor H. Smit
meta:creation-date: 2021-06-24T07:31:58Z
created: 2021-06-24T07:31:58Z
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Creation-Date: 2021-06-24T07:31:58Z
Author: Manuel Schmitz-Elbers, Gra?vydas Lukinavi?ius and Theodoor H. Smit
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dc:description: Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations.
Keywords: live fluorescence imaging; whole embryo culture; SiR-actin; F-actin
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dc:creator: Manuel Schmitz-Elbers, Gra?vydas Lukinavi?ius and Theodoor H. Smit
description: Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations.
dcterms:created: 2021-06-24T07:31:58Z
Last-Modified: 2022-04-07T11:17:20Z
dcterms:modified: 2022-04-07T11:17:20Z
title: Live Fluorescence Imaging of F-Actin Organization in Chick Whole Embryo Cultures Using SiR-Actin
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pdf:docinfo:keywords: live fluorescence imaging; whole embryo culture; SiR-actin; F-actin
pdf:docinfo:modified: 2022-04-07T11:17:20Z
meta:save-date: 2022-04-07T11:17:20Z
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creator: Manuel Schmitz-Elbers, Gra?vydas Lukinavi?ius and Theodoor H. Smit
dc:subject: live fluorescence imaging; whole embryo culture; SiR-actin; F-actin
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meta:keyword: live fluorescence imaging; whole embryo culture; SiR-actin; F-actin
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pdf:docinfo:created: 2021-06-24T07:31:58Z