date: 2023-07-05T12:41:19Z
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pdf:docinfo:title: Relevance of Host Cell Surface Glycan Structure for Cell Specificity of Influenza A Viruses
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Keywords: influenza A virus; cell binding; force spectroscopy
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subject: Influenza A viruses (IAVs) initiate infection via binding of the viral hemagglutinin (HA) to sialylated glycans on host cells. HA?s receptor specificity towards individual glycans is well studied and clearly critical for virus infection, but the contribution of the highly heterogeneous and complex glycocalyx to virus?cell adhesion remains elusive. Here, we use two complementary methods, glycan arrays and single-virus force spectroscopy (SVFS), to compare influenza virus receptor specificity with virus binding to live cells. Unexpectedly, we found that HA?s receptor binding preference does not necessarily reflect virus?cell specificity. We propose SVFS as a tool to elucidate the cell binding preference of IAVs, thereby including the complex environment of sialylated receptors within the plasma membrane of living cells.
dc:creator: Markus Kastner, Andreas Karner, Rong Zhu, Qiang Huang, Andreas Geissner, Anne Sadewasser, Markus Lesch, Xenia Wörmann, Alexander Karlas, Peter H. Seeberger, Thorsten Wolff, Peter Hinterdorfer, Andreas Herrmann and Christian Sieben
dcterms:created: 2023-07-05T12:36:49Z
Last-Modified: 2023-07-05T12:41:19Z
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title: Relevance of Host Cell Surface Glycan Structure for Cell Specificity of Influenza A Viruses
Last-Save-Date: 2023-07-05T12:41:19Z
pdf:docinfo:creator_tool: LaTeX with hyperref
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pdf:docinfo:keywords: influenza A virus; cell binding; force spectroscopy
pdf:docinfo:modified: 2023-07-05T12:41:19Z
meta:save-date: 2023-07-05T12:41:19Z
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dc:title: Relevance of Host Cell Surface Glycan Structure for Cell Specificity of Influenza A Viruses
modified: 2023-07-05T12:41:19Z
cp:subject: Influenza A viruses (IAVs) initiate infection via binding of the viral hemagglutinin (HA) to sialylated glycans on host cells. HA?s receptor specificity towards individual glycans is well studied and clearly critical for virus infection, but the contribution of the highly heterogeneous and complex glycocalyx to virus?cell adhesion remains elusive. Here, we use two complementary methods, glycan arrays and single-virus force spectroscopy (SVFS), to compare influenza virus receptor specificity with virus binding to live cells. Unexpectedly, we found that HA?s receptor binding preference does not necessarily reflect virus?cell specificity. We propose SVFS as a tool to elucidate the cell binding preference of IAVs, thereby including the complex environment of sialylated receptors within the plasma membrane of living cells.
pdf:docinfo:subject: Influenza A viruses (IAVs) initiate infection via binding of the viral hemagglutinin (HA) to sialylated glycans on host cells. HA?s receptor specificity towards individual glycans is well studied and clearly critical for virus infection, but the contribution of the highly heterogeneous and complex glycocalyx to virus?cell adhesion remains elusive. Here, we use two complementary methods, glycan arrays and single-virus force spectroscopy (SVFS), to compare influenza virus receptor specificity with virus binding to live cells. Unexpectedly, we found that HA?s receptor binding preference does not necessarily reflect virus?cell specificity. We propose SVFS as a tool to elucidate the cell binding preference of IAVs, thereby including the complex environment of sialylated receptors within the plasma membrane of living cells.
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pdf:docinfo:creator: Markus Kastner, Andreas Karner, Rong Zhu, Qiang Huang, Andreas Geissner, Anne Sadewasser, Markus Lesch, Xenia Wörmann, Alexander Karlas, Peter H. Seeberger, Thorsten Wolff, Peter Hinterdorfer, Andreas Herrmann and Christian Sieben
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creator: Markus Kastner, Andreas Karner, Rong Zhu, Qiang Huang, Andreas Geissner, Anne Sadewasser, Markus Lesch, Xenia Wörmann, Alexander Karlas, Peter H. Seeberger, Thorsten Wolff, Peter Hinterdorfer, Andreas Herrmann and Christian Sieben
meta:author: Markus Kastner, Andreas Karner, Rong Zhu, Qiang Huang, Andreas Geissner, Anne Sadewasser, Markus Lesch, Xenia Wörmann, Alexander Karlas, Peter H. Seeberger, Thorsten Wolff, Peter Hinterdorfer, Andreas Herrmann and Christian Sieben
dc:subject: influenza A virus; cell binding; force spectroscopy
meta:creation-date: 2023-07-05T12:36:49Z
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meta:keyword: influenza A virus; cell binding; force spectroscopy
Author: Markus Kastner, Andreas Karner, Rong Zhu, Qiang Huang, Andreas Geissner, Anne Sadewasser, Markus Lesch, Xenia Wörmann, Alexander Karlas, Peter H. Seeberger, Thorsten Wolff, Peter Hinterdorfer, Andreas Herrmann and Christian Sieben
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