date: 2023-08-07T03:37:16Z
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pdf:docinfo:title: Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis
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Keywords: optic neuritis; multiple sclerosis; genome-wide expression analysis
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subject: The aim of this study was to perform a genome-wide expression analysis of whole-blood samples from people with optic neuritis (ON) and to determine differentially expressed mRNAs compared to healthy control subjects. The study included eight people with acute ON and six healthy control subjects. Gene expression was analyzed using DNA microarrays for whole-human-genome analysis, which contain 54,675 25-base pairs. The additional biostatistical analysis included gene ontology analysis and gene set enrichment analysis (GSEA). Quantitative RT-PCR (qPCR) was used to confirm selected differentially expressed genes. In total, 722 differently expressed genes were identified, with 377 exhibiting increased, and 345 decreased, expression. Gene ontology analysis and GSEA revealed that protein phosphorylation and intracellular compartment, apoptosis inhibition, pathways involved in cell cycles, T and B cell functions, and anti-inflammatory central nervous system (CNS) pathways are implicated in ON pathology. qPCR confirmed the differential expression of eight selected genes, with SLPI, CR3, and ITGA4 exhibiting statistically significant results. In conclusion, whole-blood gene expression analysis showed significant differences in the expression profiles of people with ON compared to healthy control subjects. Additionally, pathways involved in T cell regulation and anti-inflammatory pathways within CNS were identified as important in the early phases of MS.
dc:creator: Mario Habek, Antonela Blazekovic, Kristina Gotovac Jercic, Nela Pivac, Tiago Fleming Outero, Fran Borovecki and Vesna Brinar
dcterms:created: 2023-08-07T03:31:45Z
Last-Modified: 2023-08-07T03:37:16Z
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title: Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis
Last-Save-Date: 2023-08-07T03:37:16Z
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pdf:docinfo:keywords: optic neuritis; multiple sclerosis; genome-wide expression analysis
pdf:docinfo:modified: 2023-08-07T03:37:16Z
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dc:title: Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis
modified: 2023-08-07T03:37:16Z
cp:subject: The aim of this study was to perform a genome-wide expression analysis of whole-blood samples from people with optic neuritis (ON) and to determine differentially expressed mRNAs compared to healthy control subjects. The study included eight people with acute ON and six healthy control subjects. Gene expression was analyzed using DNA microarrays for whole-human-genome analysis, which contain 54,675 25-base pairs. The additional biostatistical analysis included gene ontology analysis and gene set enrichment analysis (GSEA). Quantitative RT-PCR (qPCR) was used to confirm selected differentially expressed genes. In total, 722 differently expressed genes were identified, with 377 exhibiting increased, and 345 decreased, expression. Gene ontology analysis and GSEA revealed that protein phosphorylation and intracellular compartment, apoptosis inhibition, pathways involved in cell cycles, T and B cell functions, and anti-inflammatory central nervous system (CNS) pathways are implicated in ON pathology. qPCR confirmed the differential expression of eight selected genes, with SLPI, CR3, and ITGA4 exhibiting statistically significant results. In conclusion, whole-blood gene expression analysis showed significant differences in the expression profiles of people with ON compared to healthy control subjects. Additionally, pathways involved in T cell regulation and anti-inflammatory pathways within CNS were identified as important in the early phases of MS.
pdf:docinfo:subject: The aim of this study was to perform a genome-wide expression analysis of whole-blood samples from people with optic neuritis (ON) and to determine differentially expressed mRNAs compared to healthy control subjects. The study included eight people with acute ON and six healthy control subjects. Gene expression was analyzed using DNA microarrays for whole-human-genome analysis, which contain 54,675 25-base pairs. The additional biostatistical analysis included gene ontology analysis and gene set enrichment analysis (GSEA). Quantitative RT-PCR (qPCR) was used to confirm selected differentially expressed genes. In total, 722 differently expressed genes were identified, with 377 exhibiting increased, and 345 decreased, expression. Gene ontology analysis and GSEA revealed that protein phosphorylation and intracellular compartment, apoptosis inhibition, pathways involved in cell cycles, T and B cell functions, and anti-inflammatory central nervous system (CNS) pathways are implicated in ON pathology. qPCR confirmed the differential expression of eight selected genes, with SLPI, CR3, and ITGA4 exhibiting statistically significant results. In conclusion, whole-blood gene expression analysis showed significant differences in the expression profiles of people with ON compared to healthy control subjects. Additionally, pathways involved in T cell regulation and anti-inflammatory pathways within CNS were identified as important in the early phases of MS.
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pdf:docinfo:creator: Mario Habek, Antonela Blazekovic, Kristina Gotovac Jercic, Nela Pivac, Tiago Fleming Outero, Fran Borovecki and Vesna Brinar
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creator: Mario Habek, Antonela Blazekovic, Kristina Gotovac Jercic, Nela Pivac, Tiago Fleming Outero, Fran Borovecki and Vesna Brinar
meta:author: Mario Habek, Antonela Blazekovic, Kristina Gotovac Jercic, Nela Pivac, Tiago Fleming Outero, Fran Borovecki and Vesna Brinar
dc:subject: optic neuritis; multiple sclerosis; genome-wide expression analysis
meta:creation-date: 2023-08-07T03:31:45Z
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Author: Mario Habek, Antonela Blazekovic, Kristina Gotovac Jercic, Nela Pivac, Tiago Fleming Outero, Fran Borovecki and Vesna Brinar
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