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Journal Article

MINSTED tracking of single biomolecules

MPS-Authors
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Scheiderer,  Lukas
Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons15210

Hell,  Stefan W.       
Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.nature.com/articles/s41592-024-02209-6
(Any fulltext)

https://www.nature.com/articles/s41592-024-02209-6.pdf
(Any fulltext)

https://static-content.springer.com/esm/art%3A10.1038%2Fs41592-024-02209-6/MediaObjects/41592_2024_2209_MOESM1_ESM.pdf
(Supplementary material)

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Citation

Scheiderer, L., von der Emde, H., Hesselink, M., Weber, M., & Hell, S. W. (2024). MINSTED tracking of single biomolecules. Nature Methods, 21(4), 569-573. doi:10.1038/s41592-024-02209-6.


Cite as: https://hdl.handle.net/21.11116/0000-000E-B32E-5
Abstract
Here we show that MINSTED localization, a method whereby the position of a fluorophore is identified with precisely controlled beams of a STED microscope, tracks fluorophores and hence labeled biomolecules with nanometer/millisecond spatiotemporal precision. By updating the position for each detected photon, MINSTED recognizes fluorophore steps of 16 nm within <250 μs using about 13 photons. The power of MINSTED tracking is demonstrated by resolving the stepping of the motor protein kinesin-1 walking on microtubules and switching protofilaments.