High-throughput imaging of microbial biodiversity using adaptive feedback 
microscopy

Colin, S; Halavatyi, A; Zukowska, J

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High-throughput imaging of microbial biodiversity using adaptive feedback microscopy

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Colin, S
Light Microscopy, Max Planck Institute for Biology Tübingen, Max Planck Society;

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https://meetings.embo.org/files/2024-04/practicals-for-embo-webpage-(2).pdf
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引用

Colin, S., Halavatyi, A., & Zukowska, J. (2024). High-throughput imaging of microbial biodiversity using adaptive feedback microscopy. In EMBO Lecture Course: Imaging Marine Organisms Across Scales.

引用: https://hdl.handle.net/21.11116/0000-000F-217E-F

要旨

We will demonstrate the method for automated confocal imaging of a broad diversity of unicellular organisms ranging in size from 5 μm to 200μm. Cells are fixed and stained for visualising nuclei, membranes, chlorophyll and outer-surface by means of 3D-fluorescence microscopy. An adaptive feedback microscopy will be used, to set up a fully automated imaging pipeline with a high throughput. The workflow includes a low-resolution imaging of the entire preparation, the automated processing of these images for localising and selecting cells of interest, and subsequent automated triggering of slower 3D high-resolution acquisitions at relevant positions.