date: 2024-03-06T09:02:11Z
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pdf:docinfo:title: Recombinant Production of Pseudomonas aeruginosa Rhamnolipids in P. putida KT2440 on Acetobacterium woodii Cultures Grown Chemo-Autotrophically with Carbon Dioxide and Hydrogen
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Keywords: rhamnolipid; Pseudomonas putida; biosurfactants; anaerobic gas fermentation; Acetobacteriumwoodii; sequential fermentation; heterologous production
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subject: The establishment of sustainable processes for the production of commodity chemicals is one of today?s central challenges for biotechnological industries. The chemo-autotrophic fixation of CO2 and the subsequent production of acetate by acetogenic bacteria via anaerobic gas fermentation represents a promising platform for the ecologically sustainable production of high-value biocommodities via sequential fermentation processes. In this study, the applicability of acetate-containing cell-free spent medium of the gas-fermenting acetogenic bacterium A. woodii WP1 as the feeder strain for growth and the recombinant production of P. aeruginosa PAO1 mono-rhamnolipids in the well-established nonpathogenic producer strain P. putida KT2440 were investigated. Additionally, the potential possibility of a simplified production process without the necessary separation of feeder strain cells was elucidated via the cultivation of P. putida in cell-containing A. woodii culture broth. For these cultures, the content of both strains was investigated by examining the relative quantification of strain-exclusive genes via qPCR. The recombinant production of mono-rhamnolipids was successfully achieved with maximum titers of approximately 360?400 mg/L for both cell-free and cell-containing A. woodii spent medium. The reported processes therefore represent a successful proof of principle for gas fermentation-derived acetate as a potential sustainable carbon source for future recombinant rhamnolipid production processes by P. putida KT2440.
dc:creator: Jonas Widberger, Andreas Wittgens, Sebastian Klaunig, Markus Krämer, Ann-Kathrin Kissmann, Franziska Höfele, Tina Baur, Tanja Weil, Marius Henkel, Rudolf Hausmann, Frank R. Bengelsdorf, Bernhard J. Eikmanns, Peter Dürre and Frank Rosenau
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title: Recombinant Production of Pseudomonas aeruginosa Rhamnolipids in P. putida KT2440 on Acetobacterium woodii Cultures Grown Chemo-Autotrophically with Carbon Dioxide and Hydrogen
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pdf:docinfo:keywords: rhamnolipid; Pseudomonas putida; biosurfactants; anaerobic gas fermentation; Acetobacteriumwoodii; sequential fermentation; heterologous production
pdf:docinfo:modified: 2024-03-06T09:02:11Z
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dc:title: Recombinant Production of Pseudomonas aeruginosa Rhamnolipids in P. putida KT2440 on Acetobacterium woodii Cultures Grown Chemo-Autotrophically with Carbon Dioxide and Hydrogen
modified: 2024-03-06T09:02:11Z
cp:subject: The establishment of sustainable processes for the production of commodity chemicals is one of today?s central challenges for biotechnological industries. The chemo-autotrophic fixation of CO2 and the subsequent production of acetate by acetogenic bacteria via anaerobic gas fermentation represents a promising platform for the ecologically sustainable production of high-value biocommodities via sequential fermentation processes. In this study, the applicability of acetate-containing cell-free spent medium of the gas-fermenting acetogenic bacterium A. woodii WP1 as the feeder strain for growth and the recombinant production of P. aeruginosa PAO1 mono-rhamnolipids in the well-established nonpathogenic producer strain P. putida KT2440 were investigated. Additionally, the potential possibility of a simplified production process without the necessary separation of feeder strain cells was elucidated via the cultivation of P. putida in cell-containing A. woodii culture broth. For these cultures, the content of both strains was investigated by examining the relative quantification of strain-exclusive genes via qPCR. The recombinant production of mono-rhamnolipids was successfully achieved with maximum titers of approximately 360?400 mg/L for both cell-free and cell-containing A. woodii spent medium. The reported processes therefore represent a successful proof of principle for gas fermentation-derived acetate as a potential sustainable carbon source for future recombinant rhamnolipid production processes by P. putida KT2440.
pdf:docinfo:subject: The establishment of sustainable processes for the production of commodity chemicals is one of today?s central challenges for biotechnological industries. The chemo-autotrophic fixation of CO2 and the subsequent production of acetate by acetogenic bacteria via anaerobic gas fermentation represents a promising platform for the ecologically sustainable production of high-value biocommodities via sequential fermentation processes. In this study, the applicability of acetate-containing cell-free spent medium of the gas-fermenting acetogenic bacterium A. woodii WP1 as the feeder strain for growth and the recombinant production of P. aeruginosa PAO1 mono-rhamnolipids in the well-established nonpathogenic producer strain P. putida KT2440 were investigated. Additionally, the potential possibility of a simplified production process without the necessary separation of feeder strain cells was elucidated via the cultivation of P. putida in cell-containing A. woodii culture broth. For these cultures, the content of both strains was investigated by examining the relative quantification of strain-exclusive genes via qPCR. The recombinant production of mono-rhamnolipids was successfully achieved with maximum titers of approximately 360?400 mg/L for both cell-free and cell-containing A. woodii spent medium. The reported processes therefore represent a successful proof of principle for gas fermentation-derived acetate as a potential sustainable carbon source for future recombinant rhamnolipid production processes by P. putida KT2440.
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pdf:docinfo:creator: Jonas Widberger, Andreas Wittgens, Sebastian Klaunig, Markus Krämer, Ann-Kathrin Kissmann, Franziska Höfele, Tina Baur, Tanja Weil, Marius Henkel, Rudolf Hausmann, Frank R. Bengelsdorf, Bernhard J. Eikmanns, Peter Dürre and Frank Rosenau
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creator: Jonas Widberger, Andreas Wittgens, Sebastian Klaunig, Markus Krämer, Ann-Kathrin Kissmann, Franziska Höfele, Tina Baur, Tanja Weil, Marius Henkel, Rudolf Hausmann, Frank R. Bengelsdorf, Bernhard J. Eikmanns, Peter Dürre and Frank Rosenau
meta:author: Jonas Widberger, Andreas Wittgens, Sebastian Klaunig, Markus Krämer, Ann-Kathrin Kissmann, Franziska Höfele, Tina Baur, Tanja Weil, Marius Henkel, Rudolf Hausmann, Frank R. Bengelsdorf, Bernhard J. Eikmanns, Peter Dürre and Frank Rosenau
dc:subject: rhamnolipid; Pseudomonas putida; biosurfactants; anaerobic gas fermentation; Acetobacteriumwoodii; sequential fermentation; heterologous production
meta:creation-date: 2024-03-06T08:44:50Z
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meta:keyword: rhamnolipid; Pseudomonas putida; biosurfactants; anaerobic gas fermentation; Acetobacteriumwoodii; sequential fermentation; heterologous production
Author: Jonas Widberger, Andreas Wittgens, Sebastian Klaunig, Markus Krämer, Ann-Kathrin Kissmann, Franziska Höfele, Tina Baur, Tanja Weil, Marius Henkel, Rudolf Hausmann, Frank R. Bengelsdorf, Bernhard J. Eikmanns, Peter Dürre and Frank Rosenau
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