Stability of a Surface-Bound Oligonucleotide Duplex Inferred from Molecular 
Dynamics: A Study of Single Nucleotide Defects Using DNA Microarrays

Naiser, Thomas; Kayser, Jona; Mai, Timo; Michel, Wolfgang; Ott, Albrecht

doi:10.1103/PhysRevLett.102.218301

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Stability of a Surface-Bound Oligonucleotide Duplex Inferred from Molecular Dynamics: A Study of Single Nucleotide Defects Using DNA Microarrays

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https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.102.218301
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引用

Naiser, T., Kayser, J., Mai, T., Michel, W., & Ott, A. (2009). Stability of a Surface-Bound Oligonucleotide Duplex Inferred from Molecular Dynamics: A Study of Single Nucleotide Defects Using DNA Microarrays. PHYSICAL REVIEW LETTERS, 102(21):. doi:10.1103/PhysRevLett.102.218301.

引用: https://hdl.handle.net/21.11116/0000-000F-2E4D-9

要旨

Microarray technology uses the sequence dependent hybridization
(binding) affinity of surface-bound oligonucleotide strands for the
quantification of complex nucleic acid mixtures. In spite of its huge
potential in life science and medicine, microarray oligonucleotide
hybridization remains far from being understood. Taking advantage of
microarray combinatorial possibilities we show that, although surface
bound, the hybridization affinities of single-base mismatched
oligonucleotides can be derived from first principles using parameters
from bulk.