date: 2024-04-24T11:55:43Z
pdf:PDFVersion: 1.4
pdf:docinfo:title: A data-driven estimation of the ribosome drop-off rate in S. cerevisiae reveals a correlation with the genes length.
xmp:CreatorTool: OUP
access_permission:can_print_degraded: true
subject: DOI: 10.1093/nargab/lqae036 , 6, 2, 18-4-2024. Abstract: Ribosomes are the molecular machinery that catalyse all the fundamental steps involved in the translation of mRNAs into proteins. Given the complexity of this process, the efficiency of protein synthesis depends on a large number of factors among which ribosome drop-off (i.e. the premature detachment of the ribosome from the mRNA template) plays an important role. However, an in vitro quantification of the extent to which ribosome drop-off occurs is not trivial due to difficulties in obtaining the needed experimental evidence. In this work we focus on the study of ribosome drop-off in Saccharomyces cerevisiae by using ‘Ribofilio‘, a novel software tool that relies on a high sensitive strategy to estimate the ribosome drop-off rate from ribosome profiling data. Our results show that ribosome drop-off events occur at a significant rate also when S. cerevisiae is cultured in standard conditions. In this context, we also identified a correlation between the ribosome drop-off rate and the genes length: the longer the gene, the lower the drop-off rate.
language: English
dc:format: application/pdf; version=1.4
pdf:docinfo:creator_tool: OUP
access_permission:fill_in_form: true
pdf:encrypted: false
dc:title: A data-driven estimation of the ribosome drop-off rate in S. cerevisiae reveals a correlation with the genes length
modified: 2024-04-24T11:55:43Z
cp:subject: DOI: 10.1093/nargab/lqae036 , 6, 2, 18-4-2024. Abstract: Ribosomes are the molecular machinery that catalyse all the fundamental steps involved in the translation of mRNAs into proteins. Given the complexity of this process, the efficiency of protein synthesis depends on a large number of factors among which ribosome drop-off (i.e. the premature detachment of the ribosome from the mRNA template) plays an important role. However, an in vitro quantification of the extent to which ribosome drop-off occurs is not trivial due to difficulties in obtaining the needed experimental evidence. In this work we focus on the study of ribosome drop-off in Saccharomyces cerevisiae by using ‘Ribofilio‘, a novel software tool that relies on a high sensitive strategy to estimate the ribosome drop-off rate from ribosome profiling data. Our results show that ribosome drop-off events occur at a significant rate also when S. cerevisiae is cultured in standard conditions. In this context, we also identified a correlation between the ribosome drop-off rate and the genes length: the longer the gene, the lower the drop-off rate.
pdf:docinfo:subject: DOI: 10.1093/nargab/lqae036 , 6, 2, 18-4-2024. Abstract: Ribosomes are the molecular machinery that catalyse all the fundamental steps involved in the translation of mRNAs into proteins. Given the complexity of this process, the efficiency of protein synthesis depends on a large number of factors among which ribosome drop-off (i.e. the premature detachment of the ribosome from the mRNA template) plays an important role. However, an in vitro quantification of the extent to which ribosome drop-off occurs is not trivial due to difficulties in obtaining the needed experimental evidence. In this work we focus on the study of ribosome drop-off in Saccharomyces cerevisiae by using ‘Ribofilio‘, a novel software tool that relies on a high sensitive strategy to estimate the ribosome drop-off rate from ribosome profiling data. Our results show that ribosome drop-off events occur at a significant rate also when S. cerevisiae is cultured in standard conditions. In this context, we also identified a correlation between the ribosome drop-off rate and the genes length: the longer the gene, the lower the drop-off rate.
pdf:docinfo:creator: Awad Sherine, Valleriani Angelo, Chiarugi Davide
meta:author: Sherine Awad
meta:creation-date: 2024-04-18T03:58:26Z
created: 2024-04-18T03:58:26Z
access_permission:extract_for_accessibility: true
Creation-Date: 2024-04-18T03:58:26Z
pdf:docinfo:custom:doi: 10.1093/nargab/lqae036
Author: Sherine Awad
producer: Acrobat Distiller 24.0 (Windows); modified using iTextSharp 5.5.10 ©2000-2016 iText Group NV (AGPL-version)
pdf:docinfo:producer: Acrobat Distiller 24.0 (Windows); modified using iTextSharp 5.5.10 ©2000-2016 iText Group NV (AGPL-version)
doi: 10.1093/nargab/lqae036
pdf:unmappedUnicodeCharsPerPage: 0
dc:description: NAR Genomics and Bioinformatics
Keywords: 
access_permission:modify_annotations: true
dc:creator: Sherine Awad
description: NAR Genomics and Bioinformatics
dcterms:created: 2024-04-18T03:58:26Z
Last-Modified: 2024-04-24T11:55:43Z
dcterms:modified: 2024-04-24T11:55:43Z
title: A data-driven estimation of the ribosome drop-off rate in S. cerevisiae reveals a correlation with the genes length
Last-Save-Date: 2024-04-24T11:55:43Z
pdf:docinfo:keywords: 
pdf:docinfo:modified: 2024-04-24T11:55:43Z
meta:save-date: 2024-04-24T11:55:43Z
Content-Type: application/pdf
X-Parsed-By: org.apache.tika.parser.DefaultParser
creator: Sherine Awad
dc:language: English
dc:subject: 
access_permission:assemble_document: true
xmpTPg:NPages: 11
pdf:charsPerPage: 6334
access_permission:extract_content: true
access_permission:can_print: true
meta:keyword: 
access_permission:can_modify: true
pdf:docinfo:created: 2024-04-18T03:58:26Z