Recruiting the PAN2-PAN3 deadenylase complex to mRNA targets

Christie, M; Jonas, S; Boland, A; Peter, D; Huntzinger, E; Bhandari, D; Loh, B; Weichenrieder, O; Izaurralde, E

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Recruiting the PAN2-PAN3 deadenylase complex to mRNA targets

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Christie, M
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273073

Jonas, S
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons275456

Boland, A
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273095

Peter, D
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274952

Huntzinger, E
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273087

Bhandari, D
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons275626

Loh, B
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271778

Weichenrieder, O
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271767

Izaurralde, E
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Christie, M., Jonas, S., Boland, A., Peter, D., Huntzinger, E., Bhandari, D., et al. (2017). Recruiting the PAN2-PAN3 deadenylase complex to mRNA targets. In 31st Biennial Conference of the Society of Crystallographers in Australia and New Zealand (SCANZ — CRYSTAL 31) (pp. 25).

Cite as: https://hdl.handle.net/21.11116/0000-000F-3A82-D

Abstract

mRNA degradation is an essential component of the gene expression process. Cytoplasmic mRNA decay is typically initiated by the removal of poly(A) tails, a process termed deadenylation, which causes translational repression and, in most cases, triggers irreversible mRNA degradation. The PAN2- PAN3 deadenylase complex functions in both bulk and microRNA-mediated mRNA decay and is directly recruited to microRNA targets by GW182/ TNRC6 proteins. Taking a structural and functional approach, we have identified unusual features that mediate the interaction of PAN3 with PAN2 and TNRC6 proteins, as well as critical residues required for mRNA degradation in vivo. Collectively, our data describes the structural basis for the recruitment of the PAN2-PAN3 complex to microRNA targets by the TNRC6 proteins, and the essential role of PAN3 in coordinating deadenylation with downstream steps of the mRNA decay pathway.