date: 2024-05-22T07:06:45Z pdf:PDFVersion: 1.4 pdf:docinfo:title: Fungal endophytes from saline-adapted shrubs induce salinity stress tolerance in tomato seedlings xmp:CreatorTool: OUP access_permission:can_print_degraded: true subject: DOI: 10.1093/femsmc/xtae012 FEMS Microbes, 5, , 29-04-2024. Abstract: To meet the food and feed demands of the growing population, global food production needs to double by 2050. Climate change-induced challenges to food crops, especially soil salinization, remain a major threat to food production. We hypothesize that endophytic fungi isolated from salt-adapted host plants can confer salinity stress tolerance to salt-sensitive crops. Therefore, we isolated fungal endophytes from shrubs along the shores of saline alkaline Lake Magadi and evaluated their ability to induce salinity stress tolerance in tomato seeds and seedlings. Of 60 endophytic fungal isolates, 95% and 5% were from Ascomycetes and Basidiomycetes phyla, respectively. The highest number of isolates (48.3%) were from the roots. Amylase, protease and cellulase were produced by 25, 30 and 27 isolates, respectively; and 32 isolates solubilized phosphate. Only eight isolates grew at 1.5 M NaCl. Four fungal endophytes (Cephalotrichum cylindricum, Fusarium equiseti, Fusarium falciforme and Aspergilus puniceus) were tested under greenhouse conditions for their ability to induce salinity tolerance in tomato seedlings. All four endophytes successfully colonized tomato seedlings and grew in 1.5 M NaCl. The germination of endophyte-inoculated seeds was enhanced by 23%, whereas seedlings showed increased chlorophyll and biomass content and decreased hydrogen peroxide content under salinity stress, compared with controls. The results suggest that the the four isolates can potentially be used to mitigate salinity stress in tomato plants in salt-affected soils. language: English dc:format: application/pdf; version=1.4 pdf:docinfo:creator_tool: OUP access_permission:fill_in_form: true pdf:encrypted: false dc:title: Fungal endophytes from saline-adapted shrubs induce salinity stress tolerance in tomato seedlings modified: 2024-05-22T07:06:45Z cp:subject: DOI: 10.1093/femsmc/xtae012 FEMS Microbes, 5, , 29-04-2024. Abstract: To meet the food and feed demands of the growing population, global food production needs to double by 2050. Climate change-induced challenges to food crops, especially soil salinization, remain a major threat to food production. We hypothesize that endophytic fungi isolated from salt-adapted host plants can confer salinity stress tolerance to salt-sensitive crops. Therefore, we isolated fungal endophytes from shrubs along the shores of saline alkaline Lake Magadi and evaluated their ability to induce salinity stress tolerance in tomato seeds and seedlings. Of 60 endophytic fungal isolates, 95% and 5% were from Ascomycetes and Basidiomycetes phyla, respectively. The highest number of isolates (48.3%) were from the roots. Amylase, protease and cellulase were produced by 25, 30 and 27 isolates, respectively; and 32 isolates solubilized phosphate. Only eight isolates grew at 1.5 M NaCl. Four fungal endophytes (Cephalotrichum cylindricum, Fusarium equiseti, Fusarium falciforme and Aspergilus puniceus) were tested under greenhouse conditions for their ability to induce salinity tolerance in tomato seedlings. All four endophytes successfully colonized tomato seedlings and grew in 1.5 M NaCl. The germination of endophyte-inoculated seeds was enhanced by 23%, whereas seedlings showed increased chlorophyll and biomass content and decreased hydrogen peroxide content under salinity stress, compared with controls. The results suggest that the the four isolates can potentially be used to mitigate salinity stress in tomato plants in salt-affected soils. pdf:docinfo:subject: DOI: 10.1093/femsmc/xtae012 FEMS Microbes, 5, , 29-04-2024. Abstract: To meet the food and feed demands of the growing population, global food production needs to double by 2050. Climate change-induced challenges to food crops, especially soil salinization, remain a major threat to food production. We hypothesize that endophytic fungi isolated from salt-adapted host plants can confer salinity stress tolerance to salt-sensitive crops. Therefore, we isolated fungal endophytes from shrubs along the shores of saline alkaline Lake Magadi and evaluated their ability to induce salinity stress tolerance in tomato seeds and seedlings. Of 60 endophytic fungal isolates, 95% and 5% were from Ascomycetes and Basidiomycetes phyla, respectively. The highest number of isolates (48.3%) were from the roots. Amylase, protease and cellulase were produced by 25, 30 and 27 isolates, respectively; and 32 isolates solubilized phosphate. Only eight isolates grew at 1.5 M NaCl. Four fungal endophytes (Cephalotrichum cylindricum, Fusarium equiseti, Fusarium falciforme and Aspergilus puniceus) were tested under greenhouse conditions for their ability to induce salinity tolerance in tomato seedlings. All four endophytes successfully colonized tomato seedlings and grew in 1.5 M NaCl. The germination of endophyte-inoculated seeds was enhanced by 23%, whereas seedlings showed increased chlorophyll and biomass content and decreased hydrogen peroxide content under salinity stress, compared with controls. The results suggest that the the four isolates can potentially be used to mitigate salinity stress in tomato plants in salt-affected soils. pdf:docinfo:creator: Mutungi Priscillar Mumo, Wekesa Vitalis Wafula, Onguso Justus, Kanga Erustus, Baleba Steve B. S., Boga Hamadi Iddi meta:author: Mutungi Priscillar Mumo, Wekesa Vitalis Wafula, Onguso Justus, Kanga Erustus, Baleba Steve B. S., Boga Hamadi Iddi meta:creation-date: 2024-05-20T09:44:40Z created: 2024-05-20T09:44:40Z access_permission:extract_for_accessibility: true Creation-Date: 2024-05-20T09:44:40Z pdf:docinfo:custom:doi: 10.1093/femsmc/xtae012 Author: Mutungi Priscillar Mumo, Wekesa Vitalis Wafula, Onguso Justus, Kanga Erustus, Baleba Steve B. S., Boga Hamadi Iddi producer: Acrobat Distiller 24.0 (Windows); modified using iTextSharp 5.5.10 ©2000-2016 iText Group NV (AGPL-version) pdf:docinfo:producer: Acrobat Distiller 24.0 (Windows); modified using iTextSharp 5.5.10 ©2000-2016 iText Group NV (AGPL-version) doi: 10.1093/femsmc/xtae012 pdf:unmappedUnicodeCharsPerPage: 0 Keywords: access_permission:modify_annotations: true dc:creator: Mutungi Priscillar Mumo, Wekesa Vitalis Wafula, Onguso Justus, Kanga Erustus, Baleba Steve B. S., Boga Hamadi Iddi dcterms:created: 2024-05-20T09:44:40Z Last-Modified: 2024-05-22T07:06:45Z dcterms:modified: 2024-05-22T07:06:45Z title: Fungal endophytes from saline-adapted shrubs induce salinity stress tolerance in tomato seedlings Last-Save-Date: 2024-05-22T07:06:45Z pdf:docinfo:keywords: pdf:docinfo:modified: 2024-05-22T07:06:45Z meta:save-date: 2024-05-22T07:06:45Z Content-Type: application/pdf X-Parsed-By: org.apache.tika.parser.DefaultParser creator: Mutungi Priscillar Mumo, Wekesa Vitalis Wafula, Onguso Justus, Kanga Erustus, Baleba Steve B. S., Boga Hamadi Iddi dc:language: English dc:subject: X-TIKA:EXCEPTION:warn: org.xml.sax.SAXParseException; lineNumber: 5; columnNumber: 17; The prefix "pdf" for element "pdf:Keywords" is not bound. at org.apache.xerces@2.12.0.SP03//org.apache.xerces.parsers.DOMParser.parse(DOMParser.java:245) at org.apache.xerces@2.12.0.SP03//org.apache.xerces.jaxp.DocumentBuilderImpl.parse(DocumentBuilderImpl.java:298) at java.xml/javax.xml.parsers.DocumentBuilder.parse(DocumentBuilder.java:122) at deployment.pubman_ear-8.5.2.ear//org.apache.tika.utils.XMLReaderUtils.buildDOM(XMLReaderUtils.java:407) at deployment.pubman_ear-8.5.2.ear//org.apache.tika.parser.pdf.PDFParser.loadDOM(PDFParser.java:744) at deployment.pubman_ear-8.5.2.ear//org.apache.tika.parser.pdf.PDFParser.extractMetadata(PDFParser.java:234) at deployment.pubman_ear-8.5.2.ear//org.apache.tika.parser.pdf.PDFParser.parse(PDFParser.java:159) at deployment.pubman_ear-8.5.2.ear//org.apache.tika.parser.CompositeParser.parse(CompositeParser.java:280) at deployment.pubman_ear-8.5.2.ear//org.apache.tika.parser.CompositeParser.parse(CompositeParser.java:280) at deployment.pubman_ear-8.5.2.ear//org.apache.tika.parser.AutoDetectParser.parse(AutoDetectParser.java:143) at deployment.pubman_ear-8.5.2.ear//de.mpg.mpdl.inge.service.pubman.impl.FileServiceFSImpl.getFileMetadata(FileServiceFSImpl.java:449) at jdk.internal.reflect.GeneratedMethodAccessor825.invoke(Unknown Source) at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.base/java.lang.reflect.Method.invoke(Method.java:568) at deployment.pubman_ear-8.5.2.ear//org.springframework.aop.support.AopUtils.invokeJoinpointUsingReflection(AopUtils.java:344) at deployment.pubman_ear-8.5.2.ear//org.springframework.aop.framework.JdkDynamicAopProxy.invoke(JdkDynamicAopProxy.java:208) at deployment.pubman_ear-8.5.2.ear.de.mpg.mpdl.inge-pubman_presentation-8.5.2.war/jdk.proxy12/jdk.proxy12.$Proxy184.getFileMetadata(Unknown Source) at deployment.pubman_ear-8.5.2.ear.de.mpg.mpdl.inge-pubman_rest-8.5.2.war//de.mpg.mpdl.inge.rest.web.controller.ItemRestController.getTechnicalMetadataByTika(ItemRestController.java:301) at jdk.internal.reflect.GeneratedMethodAccessor824.invoke(Unknown Source) at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.base/java.lang.reflect.Method.invoke(Method.java:568) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.method.support.InvocableHandlerMethod.doInvoke(InvocableHandlerMethod.java:205) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.method.support.InvocableHandlerMethod.invokeForRequest(InvocableHandlerMethod.java:150) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.mvc.method.annotation.ServletInvocableHandlerMethod.invokeAndHandle(ServletInvocableHandlerMethod.java:117) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.mvc.method.annotation.RequestMappingHandlerAdapter.invokeHandlerMethod(RequestMappingHandlerAdapter.java:895) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.mvc.method.annotation.RequestMappingHandlerAdapter.handleInternal(RequestMappingHandlerAdapter.java:808) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.mvc.method.AbstractHandlerMethodAdapter.handle(AbstractHandlerMethodAdapter.java:87) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.DispatcherServlet.doDispatch(DispatcherServlet.java:1071) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.DispatcherServlet.doService(DispatcherServlet.java:964) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.FrameworkServlet.processRequest(FrameworkServlet.java:1006) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.FrameworkServlet.doGet(FrameworkServlet.java:898) at javax.servlet.api@2.0.0.Final//javax.servlet.http.HttpServlet.service(HttpServlet.java:503) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.servlet.FrameworkServlet.service(FrameworkServlet.java:883) at javax.servlet.api@2.0.0.Final//javax.servlet.http.HttpServlet.service(HttpServlet.java:590) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletHandler.handleRequest(ServletHandler.java:74) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.FilterHandler$FilterChainImpl.doFilter(FilterHandler.java:129) at deployment.pubman_ear-8.5.2.ear.de.mpg.mpdl.inge-pubman_rest-8.5.2.war//de.mpg.mpdl.inge.rest.web.spring.AuthCookieToHeaderFilter.doFilter(AuthCookieToHeaderFilter.java:112) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.core.ManagedFilter.doFilter(ManagedFilter.java:61) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.FilterHandler$FilterChainImpl.doFilter(FilterHandler.java:131) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.filter.CharacterEncodingFilter.doFilterInternal(CharacterEncodingFilter.java:201) at deployment.pubman_ear-8.5.2.ear//org.springframework.web.filter.OncePerRequestFilter.doFilter(OncePerRequestFilter.java:117) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.core.ManagedFilter.doFilter(ManagedFilter.java:61) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.FilterHandler$FilterChainImpl.doFilter(FilterHandler.java:131) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.FilterHandler.handleRequest(FilterHandler.java:84) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.security.ServletSecurityRoleHandler.handleRequest(ServletSecurityRoleHandler.java:62) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletChain$1.handleRequest(ServletChain.java:68) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletDispatchingHandler.handleRequest(ServletDispatchingHandler.java:36) at org.wildfly.security.elytron-web.undertow-server@1.10.1.Final//org.wildfly.elytron.web.undertow.server.ElytronRunAsHandler.lambda$handleRequest$1(ElytronRunAsHandler.java:68) at org.wildfly.security.elytron-base@1.19.1.Final//org.wildfly.security.auth.server.FlexibleIdentityAssociation.runAsFunctionEx(FlexibleIdentityAssociation.java:103) at org.wildfly.security.elytron-base@1.19.1.Final//org.wildfly.security.auth.server.Scoped.runAsFunctionEx(Scoped.java:161) at org.wildfly.security.elytron-base@1.19.1.Final//org.wildfly.security.auth.server.Scoped.runAs(Scoped.java:73) at org.wildfly.security.elytron-web.undertow-server@1.10.1.Final//org.wildfly.elytron.web.undertow.server.ElytronRunAsHandler.handleRequest(ElytronRunAsHandler.java:67) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.RedirectDirHandler.handleRequest(RedirectDirHandler.java:68) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.security.SSLInformationAssociationHandler.handleRequest(SSLInformationAssociationHandler.java:117) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.security.ServletAuthenticationCallHandler.handleRequest(ServletAuthenticationCallHandler.java:57) at io.undertow.core@2.2.19.Final//io.undertow.server.handlers.PredicateHandler.handleRequest(PredicateHandler.java:43) at io.undertow.core@2.2.19.Final//io.undertow.security.handlers.AbstractConfidentialityHandler.handleRequest(AbstractConfidentialityHandler.java:46) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.security.ServletConfidentialityConstraintHandler.handleRequest(ServletConfidentialityConstraintHandler.java:64) at io.undertow.core@2.2.19.Final//io.undertow.security.handlers.AbstractSecurityContextAssociationHandler.handleRequest(AbstractSecurityContextAssociationHandler.java:43) at org.wildfly.security.elytron-web.undertow-server-servlet@1.10.1.Final//org.wildfly.elytron.web.undertow.server.servlet.CleanUpHandler.handleRequest(CleanUpHandler.java:38) at io.undertow.core@2.2.19.Final//io.undertow.server.handlers.PredicateHandler.handleRequest(PredicateHandler.java:43) at org.wildfly.extension.undertow@26.1.3.Final//org.wildfly.extension.undertow.security.jacc.JACCContextIdHandler.handleRequest(JACCContextIdHandler.java:61) at io.undertow.core@2.2.19.Final//io.undertow.server.handlers.PredicateHandler.handleRequest(PredicateHandler.java:43) at org.wildfly.extension.undertow@26.1.3.Final//org.wildfly.extension.undertow.deployment.GlobalRequestControllerHandler.handleRequest(GlobalRequestControllerHandler.java:68) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.SendErrorPageHandler.handleRequest(SendErrorPageHandler.java:52) at io.undertow.core@2.2.19.Final//io.undertow.server.handlers.PredicateHandler.handleRequest(PredicateHandler.java:43) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletInitialHandler.handleFirstRequest(ServletInitialHandler.java:275) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletInitialHandler.access$100(ServletInitialHandler.java:79) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletInitialHandler$2.call(ServletInitialHandler.java:134) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletInitialHandler$2.call(ServletInitialHandler.java:131) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.core.ServletRequestContextThreadSetupAction$1.call(ServletRequestContextThreadSetupAction.java:48) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.core.ContextClassLoaderSetupAction$1.call(ContextClassLoaderSetupAction.java:43) at org.wildfly.extension.undertow@26.1.3.Final//org.wildfly.extension.undertow.deployment.UndertowDeploymentInfoService$UndertowThreadSetupAction.lambda$create$0(UndertowDeploymentInfoService.java:1544) at org.wildfly.extension.undertow@26.1.3.Final//org.wildfly.extension.undertow.deployment.UndertowDeploymentInfoService$UndertowThreadSetupAction.lambda$create$0(UndertowDeploymentInfoService.java:1544) at org.wildfly.extension.undertow@26.1.3.Final//org.wildfly.extension.undertow.deployment.UndertowDeploymentInfoService$UndertowThreadSetupAction.lambda$create$0(UndertowDeploymentInfoService.java:1544) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletInitialHandler.dispatchRequest(ServletInitialHandler.java:255) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletInitialHandler.access$000(ServletInitialHandler.java:79) at io.undertow.servlet@2.2.19.Final//io.undertow.servlet.handlers.ServletInitialHandler$1.handleRequest(ServletInitialHandler.java:100) at io.undertow.core@2.2.19.Final//io.undertow.server.Connectors.executeRootHandler(Connectors.java:387) at io.undertow.core@2.2.19.Final//io.undertow.server.HttpServerExchange$1.run(HttpServerExchange.java:852) at org.jboss.threads@2.4.0.Final//org.jboss.threads.ContextClassLoaderSavingRunnable.run(ContextClassLoaderSavingRunnable.java:35) at org.jboss.threads@2.4.0.Final//org.jboss.threads.EnhancedQueueExecutor.safeRun(EnhancedQueueExecutor.java:1990) at org.jboss.threads@2.4.0.Final//org.jboss.threads.EnhancedQueueExecutor$ThreadBody.doRunTask(EnhancedQueueExecutor.java:1486) at org.jboss.threads@2.4.0.Final//org.jboss.threads.EnhancedQueueExecutor$ThreadBody.run(EnhancedQueueExecutor.java:1377) at org.jboss.xnio@3.8.7.Final//org.xnio.XnioWorker$WorkerThreadFactory$1$1.run(XnioWorker.java:1282) at java.base/java.lang.Thread.run(Thread.java:840) access_permission:assemble_document: true xmpTPg:NPages: 17 pdf:charsPerPage: 6506 access_permission:extract_content: true access_permission:can_print: true meta:keyword: access_permission:can_modify: true pdf:docinfo:created: 2024-05-20T09:44:40Z