A prion-like domain is required for phase separation and chloroplast RNA 
processing during cold acclimation in Arabidopsis

Legen, Julia; Lenzen, Benjamin; Kachariya, Nitin; Feltgen, Stephanie; Gao, Y.; Mergenthal, Simon; Weber, Willi; Klotzsch, Enrico; Zoschke, R.; Sattler, Michael; Schmitz-Linneweber, Christian

doi:.1093/plcell/koae145

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A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis

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Gao, Y.
Translational Regulation in Plants, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Zoschke, R.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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引用

Legen, J., Lenzen, B., Kachariya, N., Feltgen, S., Gao, Y., Mergenthal, S., Weber, W., Klotzsch, E., Zoschke, R., Sattler, M., & Schmitz-Linneweber, C. (2024). A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis. The Plant Cell,. doi:.1093/plcell/koae145.

引用: https://hdl.handle.net/21.11116/0000-000F-4432-C

要旨

Arabidopsis (Arabidopsis thaliana) plants can produce photosynthetic tissue with active chloroplasts at temperatures as low as 4°C, and this process depends on the presence of the nuclear-encoded, chloroplast-localized RNA-binding protein CP29A. In this study, we demonstrate that CP29A undergoes phase separation in vitro and in vivo in a temperature-dependent manner, which is mediated by a prion-like domain (PLD) located between the two RNA recognition motif (RRM) domains of CP29A. The resulting droplets display liquid-like properties and are found near chloroplast nucleoids. The PLD is required to support chloroplast RNA splicing and translation in cold-treated tissue. Together, our findings suggest that plant chloroplast gene expression is compartmentalized by inducible condensation of CP29A at low temperatures, a mechanism that could play a crucial role in plant cold resistance.