Imaging bioluminescence by detecting localized haemodynamic contrast from 
photosensitized vasculature

Ohlendorf, R; Li, N; Van, VDP; Schwalm, M; Ke, Y; Dawson, M; Jiang, Y; Das, S; Stallings, B; Zheng, WT; Jasanoff, A

doi:10.1038/s41551-024-01210-w

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

前へ次へ / 524933

公開

学術論文

Imaging bioluminescence by detecting localized haemodynamic contrast from photosensitized vasculature

MPS-Authors

/persons/resource/persons287630

Ohlendorf, R
Research Group Molecular Signaling, Max Planck Institute for Biological Cybernetics, Max Planck Society;

External Resource

https://www.nature.com/articles/s41551-024-01210-w.pdf
(出版社版)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Ohlendorf, R., Li, N., Van, V., Schwalm, M., Ke, Y., Dawson, M., Jiang, Y., Das, S., Stallings, B., Zheng, W., & Jasanoff, A. (2024). Imaging bioluminescence by detecting localized haemodynamic contrast from photosensitized vasculature. Nature Biomedical Engineering, Epub ahead. doi:10.1038/s41551-024-01210-w.

引用: https://hdl.handle.net/21.11116/0000-000F-5034-C

要旨

Bioluminescent probes are widely used to monitor biomedically relevant processes and cellular targets in living animals. However, the absorption and scattering of visible light by tissue drastically limit the depth and resolution of the detection of luminescence. Here we show that bioluminescent sources can be detected with magnetic resonance imaging by leveraging the light-mediated activation of vascular cells expressing a photosensitive bacterial enzyme that causes the conversion of bioluminescent emission into local changes in haemodynamic contrast. In the brains of rats with photosensitized vasculature, we used magnetic resonance imaging to volumetrically map bioluminescent xenografts and cell populations virally transduced to express luciferase. Detecting bioluminescence-induced haemodynamic signals from photosensitized vasculature will extend the applications of bioluminescent probes.