アイテム詳細

要旨

Liquid-jet photoemission spectroscopy (LJ-PES) allows for a direct probing of electronic structure in aqueous solutions. We show the applicability of the approach to biomolecules in a complex environment, exploring site-specific information on the interaction of adenosine triphosphate in the aqueous phase (ATP(aq)) with magnesium (Mg²⁺_(aq)), highlighting the synergy brought about by the simultaneous analysis of different regions in the photoelectron spectrum. In particular, we demonstrate intermolecular Coulombic decay (ICD) spectroscopy as a new and powerful addition to the arsenal of techniques for biomolecular structure investigation. We apply LJ-PES assisted by electronic-structure calculations to study ATP_(aq) solutions with and without dissolved Mg²⁺. Valence photoelectron data reveal spectral changes in the phosphate and adenine features of ATP_(aq) due to interactions with the divalent cation. Chemical shifts in Mg 2p, Mg 2s, P 2p, and P 2s core-level spectra as a function of the Mg²⁺/ATP concentration ratio are correlated to the formation of [Mg(ATP)₂]^6-_(aq), [MgATP]^2-_(aq), and [Mg₂ATP]_(aq)complexes, demonstrating the element sensitivity of the technique to Mg²⁺–phosphate interactions. The most direct probe of the intermolecular interactions between ATP_(aq) and Mg²⁺_(aq) is delivered by the emerging ICD electrons following ionization of Mg 1s electrons. ICD spectra are shown to sensitively probe ligand exchange in theMg²⁺–ATP_(aq) coordination environment. In addition, we report and compare P 2s data from ATP_(aq) and adenosine mono- and diphosphate (AMP_(aq) and ADP_(aq), respectively) solutions, probing the electronic structure of the phosphate chain and the local environment of individual phosphate units in ATP_(aq). Our results provide a comprehensive view of the electronic structure of ATP_(aq) and Mg²⁺–ATP_(aq) complexes relevant to phosphorylation and dephosphorylation reactions that are central to bioenergetics in living organisms.