FACT maintains chromatin architecture and thereby stimulates RNA polymerase II 
pausing during transcription in vivo

Žumer, Kristina; Ochmann, Moritz; Aljahani, Abrar; Zheenbekova, Aiturgan; Devadas, Arjun; Maier, Kerstin C.; Rus, Petra; Neef, Ute; Oudelaar, Aukje Marieke; Cramer, Patrick

doi:10.1016/j.molcel.2024.05.003

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学術論文

FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo

MPS-Authors

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Žumer, Kristina
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons248419

Ochmann, Moritz
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons252095

Aljahani, Abrar
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons298756

Zheenbekova, Aiturgan
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons298758

Devadas, Arjun
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons199094

Maier, Kerstin C.
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons203891

Rus, Petra
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons252091

Oudelaar, Aukje Marieke
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons127020

Cramer, Patrick
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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引用

Žumer, K., Ochmann, M., Aljahani, A., Zheenbekova, A., Devadas, A., Maier, K. C., Rus, P., Neef, U., Oudelaar, A. M., & Cramer, P. (2024). FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo. Molecular Cell, 84(11), 2053-2069.e9. doi:10.1016/j.molcel.2024.05.003.

引用: https://hdl.handle.net/21.11116/0000-000F-5BF8-4

要旨

Facilitates chromatin transcription (FACT) is a histone chaperone that supports transcription through chromatin in vitro, but its functional roles in vivo remain unclear. Here, we analyze the in vivo functions of FACT with the use of multi-omics analysis after rapid FACT depletion from human cells. We show that FACT depletion destabilizes chromatin and leads to transcriptional defects, including defective promoter-proximal pausing and elongation, and increased premature termination of RNA polymerase II. Unexpectedly, our analysis revealed that promoter-proximal pausing depends not only on the negative elongation factor (NELF) but also on the +1 nucleosome, which is maintained by FACT.