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Journal Article

Real-Time Metabolic Magnetic Resonance Spectroscopy of Pancreatic and Colon Cancer Tumor-Xenografts with Parahydrogen Hyperpolarized 1-13C Pyruvate-d3

MPS-Authors
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Fries,  Lisa
Research Group of NMR Signal Enhancement, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Hune,  Theresa L. K.
Research Group of NMR Signal Enhancement, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Sternkopf,  Sonja
Research Group of NMR Signal Enhancement, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Mamone,  Salvatore
Research Group of NMR Signal Enhancement, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Becker,  Dorothea
Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Glöggler,  Stefan
Research Group of NMR Signal Enhancement, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Citation

Fries, L., Hune, T. L. K., Sternkopf, S., Mamone, S., Schneider, K. L., Schulz-Heddergott, R., et al. (2024). Real-Time Metabolic Magnetic Resonance Spectroscopy of Pancreatic and Colon Cancer Tumor-Xenografts with Parahydrogen Hyperpolarized 1-13C Pyruvate-d3. Chemistry – A European Journal. doi:10.1002/chem.202400187.


Cite as: https://hdl.handle.net/21.11116/0000-000F-7923-2
Abstract
Parahydrogen-induced polarization (PHIP) is an emerging technique to enhance the signal of stable isotope metabolic contrast agents for Magnetic Resonance (MR). The objective of this study is to continue establishing 1-13C-pyruvate-d3, signal-enhanced via PHIP, as a hyperpolarized contrast agent, obtained in seconds, to monitor metabolism in human cancer. Our focus was on human pancreatic and colon tumor xenografts. 1-13C-vinylpyruvate-d6 was hydrogenated using parahydrogen. Thereafter, the polarization of the protons was transferred to 13C. Following a workup procedure, the free hyperpolarized 1-13C-pyruvate-d3 was obtained in clean aqueous solution. After injection into animals bearing either pancreatic or colon cancer xenografts, slice-selective MR spectra were acquired and analyzed to determine rate constants of metabolic conversion into lactate and alanine. 1-13C-pyruvate-d3 proved to follow the increased metabolic rate to lactate and alanine in the tumor xenografts.