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Journal Article

Simultaneous multicolor fluorescence imaging using PSF splitting

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Auer,  Alexander
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Hellmeier,  Joschka
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Schlichthärle,  Thomas
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons172959

Jungmann,  Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Van den Eynde, R., Hertel, F., Abakumov, S., Krajnik, B., Hugelier, S., Auer, A., et al. (2024). Simultaneous multicolor fluorescence imaging using PSF splitting. Nature Methods. doi:10.1038/s41592-024-02383-7.


Cite as: https://hdl.handle.net/21.11116/0000-000F-DD65-7
Abstract
We present a way to encode more information in fluorescence imaging by splitting the original point spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the 'Circulator', an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view.
Point spread function (PSF) splitting with the 'Circulator', which encodes the fluorophore emission band into the PSF, improves the information content of fluorescence microscopy and enables improved super-resolution imaging and single-particle tracking.