The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for 
Recombinant Protein Production.

Flock, Julia; Xie, Yexin; Lemaitre, Regis P.; Lapouge, Karine; Remans, Kim

doi:10.1007/978-1-0716-3878-1_3

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben

Zeitschriftenartikel

The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production.

MPG-Autoren

/persons/resource/persons219380

Lemaitre, Regis P.
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

Externe Ressourcen

Es sind keine externen Ressourcen hinterlegt

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

Es sind keine frei zugänglichen Volltexte in PuRe verfügbar

Ergänzendes Material (frei zugänglich)

Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar

Zitation

Flock, J., Xie, Y., Lemaitre, R. P., Lapouge, K., & Remans, K. (2024). The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production. Methods in molecular biology (Clifton, N.J.), 2810, 29-53. doi:10.1007/978-1-0716-3878-1_3.

Zitierlink: https://hdl.handle.net/21.11116/0000-0010-D5AF-9

Zusammenfassung

Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production in various types of mammalian cell lines. We decided to establish BacMam in our lab in order to streamline our workflows for gene expression in insect and mammalian cells, as it is straightforward to parallelize the baculovirus generation for both types of eukaryotic cells. This chapter provides a step-by-step description of the protocols we use for the generation of the recombinant BacMam viruses, the transduction of mammalian cell cultures, and optimization of the protein production conditions through small-scale expression and purification tests.