Combining deconvolution and fluctuation analysis to determine quantal 
parameters and release rates

Neher, E.; Sakaba, T.

doi:10.1016/j.jneumeth.2003.09.020

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Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates

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Neher, E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Sakaba, T.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Neher, E., & Sakaba, T. (2003). Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates. Journal of Neuroscience Methods, 130(2), 143-157. doi:10.1016/j.jneumeth.2003.09.020.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-EF7A-E

Abstract

Analysis methods are described which integrate information from fluctuation analysis with that from deconvolution. Together the two approaches allow to derive a consistent quantitative description of quantal release (both evoked, spontaneous and asynchronous) under conditions in which quantal parameters may change during a repetitively applied stimulation protocol. Specifically, our methods take into account the effects of accumulating transmitter in the synaptic cleft and postsynaptic receptor desensitization, which may develop during strong stimulation. Several ways to handle non-stationarities are described. Examples are provided for the Calyx of Held, a glutamatergic synapse, in which both the pre- and the postsynaptic compartments can be voltage-clamped.