Supplementary Figure 6: Finding optimal conditions for reconstitution experiments.
From ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space
- Ashwin Chari1, n1
- David Haselbach1, n1
- Jan-Martin Kirves1, n1
- Juergen Ohmer2,
- Elham Paknia2,
- Niels Fischer1,
- Oleg Ganichkin3,
- Vanessa Möller4,
- Jeremiah J Frye5,
- Georg Petzold6,
- Marc Jarvis6,
- Michael Tietzel7,
- Clemens Grimm2,
- Jan-Michael Peters6,
- Brenda A Schulman5, 8,
- Kai Tittmann7,
- Jürgen Markl4,
- Utz Fischer2,
- Holger Stark1,
- Journal name:
- Nature Methods
- Volume:
- 12,
- Pages:
- 859–865
- Year published:
- DOI:
- doi:10.1038/nmeth.3493
- Received
- Accepted
- Published online
Additional data
Author footnotes
These authors contributed equally to this work.
- Ashwin Chari,
- David Haselbach &
- Jan-Martin Kirves
Affiliations
-
Research Group of 3D Electron Cryomicroscopy, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
- Ashwin Chari,
- David Haselbach,
- Jan-Martin Kirves,
- Niels Fischer &
- Holger Stark
-
Department of Biochemistry, Theodor-Boveri Institute, University of Würzburg, Würzburg, Germany.
- Juergen Ohmer,
- Elham Paknia,
- Clemens Grimm &
- Utz Fischer
-
Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany.
- Oleg Ganichkin
-
Institut für Zoologie – Abteilung für Molekular Tierphysiologie, Johannes Gutenberg Universität Mainz, Mainz, Germany.
- Vanessa Möller &
- Jürgen Markl
-
Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
- Jeremiah J Frye &
- Brenda A Schulman
-
Research Institute of Molecular Pathology, Vienna, Austria.
- Georg Petzold,
- Marc Jarvis &
- Jan-Michael Peters
-
Department for Bioanalytics, Georg-August University Göttingen, Göttingen, Germany.
- Michael Tietzel &
- Kai Tittmann
-
Howard Hughes Medical Institute, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
- Brenda A Schulman
Contributions
A.C. and H.S. designed research; A.C. and D.H. performed most experiments and data analysis with the support of J.O., E.P., O.G. and V.M.; D.H. derived the thermodynamic theory used for the analysis; J.-M.K. designed an analysis software and performed analysis of data; N.F., C.G., J.J.F., M.J., M.T. and G.P. performed further experiments; J.M., J.-M.P., B.A.S. and K.T. supervised research partially; initial experiments were performed in the lab of U.F.; A.C., D.H., J.-M.K. and H.S. analyzed the data and prepared the manuscript.
Competing financial interests
A.C., D.H., J.-M.K. and H.S. have filed a patent application (WO2013034160 A1: “Methods for analyzing biological macromolecular complexes and use thereof”) and are currently developing a relevant product in cooperation with FEI Company.
Author details
Ashwin Chari
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David Haselbach
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Jan-Martin Kirves
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Juergen Ohmer
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Elham Paknia
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Niels Fischer
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Oleg Ganichkin
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Vanessa Möller
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Jeremiah J Frye
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Georg Petzold
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Marc Jarvis
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Michael Tietzel
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Clemens Grimm
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Jan-Michael Peters
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Brenda A Schulman
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Kai Tittmann
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Jürgen Markl
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Utz Fischer
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Holger Stark
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Supplementary Figure 1: Data analysis workflow.Hover over figure to zoom
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Supplementary Figure 2: Schematic representation of possible unfolding scenarios.Hover over figure to zoom
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Supplementary Figure 3: Theoretical unfolding transition behaviors.Hover over figure to zoom
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Supplementary Figure 4: Evaluation of the quality of ProteoPlex data approximation.Hover over figure to zoom
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Supplementary Figure 5: Screening for additives.Hover over figure to zoom
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Supplementary Figure 6: Finding optimal conditions for reconstitution experiments.Hover over figure to zoom
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Supplementary Figure 7: Automation.Hover over figure to zoom
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Supplementary Figure 8: Test of different real-time PCR (RT-PCR) machines.Hover over figure to zoom