Video 1: DUSP7 is required to release oocytes from prophase arrest
FromLive imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes
- Sybille Pfender1, *
- Vitaliy Kuznetsov1, *
- Michał Pasternak1, *
- Thomas Tischer1,
- Balaji Santhanam1,
- Melina Schuh1,
- Journal name:
- Nature
- Volume:
- 524,
- Pages:
- 239–242
- Date published:
- DOI:
- doi:10.1038/nature14568
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Live oocytes microinjected with control siRNA (Control) or siRNAs targeting Dusp7 alone (Dusp7 RNAi) or with mRNA encoding EGFP-DUSP7, H2B-mRFP (magenta, chromosomes) merged with DIC.More videos from this article
Additional data
Author footnotes
These authors contributed equally to this work.
- Sybille Pfender,
- Vitaliy Kuznetsov &
- Michał Pasternak
Affiliations
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Medical Research Council, Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK
- Sybille Pfender,
- Vitaliy Kuznetsov,
- Michał Pasternak,
- Thomas Tischer,
- Balaji Santhanam &
- Melina Schuh
Contributions
All authors analysed data. S.P. microinjected the majority of siRNA mixes, and identified, validated and characterized most genes; M.P. microinjected siRNA mixes, compared the developmental capacity and expression profile of oocytes grown in vitro and in vivo, and identified, validated and characterized several genes; V.K. microinjected siRNA mixes and wrote software in OriginPro to quantify phenotypes; T.T. validated and characterized Dusp7; B.S. did all bioinformatics analyses; V.K. and M.S. developed and established the strategy of the screen; M.S. wrote the manuscript; S.P., M.P., T.T. and B.S. commented on and edited the manuscript; M.P. and M.S. prepared the revised manuscript; M.S. supervised the study.
Competing financial interests
The authors declare no competing financial interests.
RNA sequencing data have been deposited in Gene Expression Omnibus under accession number GSE68150.
Author details
Sybille Pfender
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Vitaliy Kuznetsov
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Michał Pasternak
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Thomas Tischer
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Balaji Santhanam
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Melina Schuh
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Extended Data Figure 1: Efficiency of follicle growth and comparison of oocytes grown in vitro and in vivo.Hover over figure to zoom
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Extended Data Figure 2: Transcriptome analysis of oocytes grown in vivo and in vitro.Hover over figure to zoom
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Extended Data Figure 3: Description of defects scored in screen.Hover over figure to zoom
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Extended Data Figure 4: Defects during meiosis II in siRNA-treated oocytes.Hover over figure to zoom
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Extended Data Figure 5: Frequency of meiosis I and meiosis II defects in oocytes treated with control siRNAs.Hover over figure to zoom
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Extended Data Figure 6: Eif4enif1 is required for release from prophase arrest and Birc5 for spindle integrity.Hover over figure to zoom
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Extended Data Figure 7: Aspm function in mouse oocytes.Hover over figure to zoom
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Extended Data Figure 8: Mastl is required for metaphase II arrest and accurate chromosome segregation, but is dispensable for cytokinesis and chromosome condensation in mouse oocytes.Hover over figure to zoom
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Extended Data Figure 9: Systematic analysis of phenotype correlations in mouse oocytes.Hover over figure to zoom
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Extended Data Figure 10: Network of phenotypes and calculation of Jaccard indices.Hover over figure to zoom
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Video 1: DUSP7 is required to release oocytes from prophase arrest
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Video 2: ASPM is required to prevent lagging chromosomes in oocytes