Streaming video does not reproduce the original video quality. Download the video using the link below to view at original quality.Live oocytes microinjected with control siRNA (Control) or siRNAs targeting Aspm (Aspm RNAi) expressing mEGFP-α-tubulin (green, microtubules) and H2B-mRFP (magenta, chromosomes).
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These authors contributed equally to this work.
- Sybille Pfender,
- Vitaliy Kuznetsov &
- Michał Pasternak
Medical Research Council, Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK
- Sybille Pfender,
- Vitaliy Kuznetsov,
- Michał Pasternak,
- Thomas Tischer,
- Balaji Santhanam &
- Melina Schuh
All authors analysed data. S.P. microinjected the majority of siRNA mixes, and identified, validated and characterized most genes; M.P. microinjected siRNA mixes, compared the developmental capacity and expression profile of oocytes grown in vitro and in vivo, and identified, validated and characterized several genes; V.K. microinjected siRNA mixes and wrote software in OriginPro to quantify phenotypes; T.T. validated and characterized Dusp7; B.S. did all bioinformatics analyses; V.K. and M.S. developed and established the strategy of the screen; M.S. wrote the manuscript; S.P., M.P., T.T. and B.S. commented on and edited the manuscript; M.P. and M.S. prepared the revised manuscript; M.S. supervised the study.
Competing financial interests
The authors declare no competing financial interests.
RNA sequencing data have been deposited in Gene Expression Omnibus under accession number GSE68150.
Extended Data Figure 1: Efficiency of follicle growth and comparison of oocytes grown in vitro and in vivo.Hover over figure to zoom
Extended Data Figure 2: Transcriptome analysis of oocytes grown in vivo and in vitro.Hover over figure to zoom
Extended Data Figure 3: Description of defects scored in screen.Hover over figure to zoom
Extended Data Figure 4: Defects during meiosis II in siRNA-treated oocytes.Hover over figure to zoom
Extended Data Figure 5: Frequency of meiosis I and meiosis II defects in oocytes treated with control siRNAs.Hover over figure to zoom
Extended Data Figure 6: Eif4enif1 is required for release from prophase arrest and Birc5 for spindle integrity.Hover over figure to zoom
Extended Data Figure 7: Aspm function in mouse oocytes.Hover over figure to zoom
Extended Data Figure 8: Mastl is required for metaphase II arrest and accurate chromosome segregation, but is dispensable for cytokinesis and chromosome condensation in mouse oocytes.Hover over figure to zoom
Extended Data Figure 9: Systematic analysis of phenotype correlations in mouse oocytes.Hover over figure to zoom
Extended Data Figure 10: Network of phenotypes and calculation of Jaccard indices.Hover over figure to zoom
Video 1: DUSP7 is required to release oocytes from prophase arrest
Video 2: ASPM is required to prevent lagging chromosomes in oocytes