Supplementary Figure 4: Quality of the electron density.
From The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease
- Daniela Lazzaretti1,
- Katharina Veith1, n1
- Katharina Kramer2, 3, n1
- Claire Basquin4,
- Henning Urlaub2, 3,
- Uwe Irion1,
- Fulvia Bono1,
- Journal name:
- Nature Structural & Molecular Biology
- Volume:
- 23,
- Pages:
- 705–713
- Year published:
- DOI:
- doi:10.1038/nsmb.3254
- Received
- Accepted
- Published online
Additional data
Author footnotes
Present addresses: Institute for Biochemistry and Molecular Biology, University of Hamburg, Hamburg, Germany (K.V.) and Max Planck Institute for Plant Breeding Research, Köln, Germany (K.K.).
- Katharina Veith &
- Katharina Kramer
Affiliations
-
Max Planck Institute for Developmental Biology, Tübingen, Germany.
- Daniela Lazzaretti,
- Katharina Veith,
- Uwe Irion &
- Fulvia Bono
-
Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
- Katharina Kramer &
- Henning Urlaub
-
Bioanalytics, Institute for Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany.
- Katharina Kramer &
- Henning Urlaub
-
Max Planck Institute of Biochemistry, Martinsried, Germany.
- Claire Basquin
Contributions
Biochemical, biophysical and crystallization work was performed by D.L. and K.V.; fly work was performed by D.L. and U.I.; K.K. and H.U. carried out the MS analysis; D.L. and C.B. analyzed FA data; F.B. solved the structures and supervised the project. F.B., D.L. and U.I. wrote the paper.
Competing financial interests
The authors declare no competing financial interests.
Author details
Daniela Lazzaretti
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Katharina Veith
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Katharina Kramer
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Claire Basquin
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Henning Urlaub
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Uwe Irion
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Fulvia Bono
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Supplementary Figure 1: Alignment of Exu homologs.Hover over figure to zoom
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Supplementary Figure 2: Structural homologs of the Exu SAM-like domain.Hover over figure to zoom
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Supplementary Figure 3: Comparison of DEDD exo- and pseudonucleases.Hover over figure to zoom
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Supplementary Figure 4: Quality of the electron density.Hover over figure to zoom
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Supplementary Figure 5: Exu and Vts1p SAM domains use different surfaces for RNA binding.Hover over figure to zoom
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Supplementary Figure 6: Exu can bind two RNA molecules.Hover over figure to zoom
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Supplementary Figure 7: Localization of Venus-tagged Exu transgenes.Hover over figure to zoom