Video 4: Supplementary Video 4
FromEngineered non-Mendelian inheritance of entire parental genomes in C. elegans
- Journal name:
- Nature Biotechnology
- Volume:
- 34,
- Pages:
- 982–986
- Year published:
- DOI:
- doi:10.1038/nbt.3643
- Received
- Accepted
- Published online
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An example of segregation of the paternal chromosomes to the P lineage. Anterior is to the left. The paternal pronucleus can be formally identified because it is located further to the posterior and has the two centrosomes attached. The maternal pronucleus is located initially in the anterior, has no centrosomes attached and is more mobile. Thus, the two pronuclei meet in the posterior to form the PCC. The PCC is then pulled apart so that the maternal pronucleus forms a monopolar spindle in the anterior, and the paternal pronucleus forms a monopolar spindle in the posterior. Frames were taken every 20 seconds and the display rate is 3 frames per second.More videos from this article
Additional data
Affiliations
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Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
- Judith Besseling &
- Henrik Bringmann
Contributions
J.B. performed experiments, analyzed data, and edited the manuscript. H.B. conceived the study, performed experiments, analyzed data, and wrote the manuscript.
Competing financial interests
The authors declare no competing financial interests.
Author details
Judith Besseling
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Henrik Bringmann
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Supplementary Figure 1: Overexpression of YFP::GPR-1 in the germline.Hover over figure to zoom
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Supplementary Figure 2: Fluorescence imaging of a control embryo that did not overexpress GPR-1.Hover over figure to zoom
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Supplementary Figure 3: Fluorescence imaging of a GPR-1 overexpressing embryo that formed a bipolar spindle.Hover over figure to zoom
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Supplementary Figure 4: Fluorescence imaging of a GPR-1 overexpressing embryo that formed two monopolar spindles in the zygote.Hover over figure to zoom
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Supplementary Figure 5: Despite forming two monopolar spindles in the P0 zygote, embryos formed normal bipolar spindles in subsequent divisions.Hover over figure to zoom
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Supplementary Figure 6: Some zygotes that formed two monopolar spindles survived after imaging and recovery.Hover over figure to zoom
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Supplementary Figure 7: Following unequal chromosome segregation into hypodermal cells.Hover over figure to zoom
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Supplementary Figure 8: Following unequal chromosome segregation into neurons.Hover over figure to zoom
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Supplementary Figure 9: Following unequal chromosome segregation into cells of the pharynx and body wall muscles.Hover over figure to zoom
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Supplementary Figure 10: Following the segregation of maternal DNA into the P lineage.Hover over figure to zoom
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Supplementary Figure 11: Crossing red GPR-1 OE hermaphrodites with green males resulted in two types of cross-progeny: F1 that co-expressed both markers, and F1 that expressed the markers in separate tissues.Hover over figure to zoom
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Supplementary Figure 12: Non-Mendelian genetics: Following the transmission of recessive alleles from F1 to F2 (scoring of F2 broods derived from co-expressing F1)Hover over figure to zoom
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Supplementary Figure 13: Non-Mendelian genetics: Following the transmission of recessive alleles from F1 to F2 (scoring of F2 broods derived from separately expressing F1)Hover over figure to zoom
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Video 1: Supplementary Video 1
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Video 2: Supplementary Video 2
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Video 3: Supplementary Video 3
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Video 4: Supplementary Video 4
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Video 5: Supplementary Video 5