Supplementary Materials

Actin protects mammalian eggs against chromosome segregation errors

Binyam Mogessie and Melina Schuh

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Movies S1 to S13

Images, Video, and Other Media

Movie S1
Time lapse movie of spindle actin during anaphase I in a mouse oocyte microinjected with EGFP-UtrCH (to label actin) and H2B-mRFP (to label chromosomes)
Movie S2
Time lapse movie of spindle actin during meiosis I spindle relocation in a mouse oocyte microinjected with EGFP-UtrCH (to label actin) and H2B-mRFP (to label chromosomes)
Movie S3
Super-resolution (Zeiss Airyscan) time lapse movie of spindle microtubule depolymerization by Nocodazole in a mouse egg. SNAP-MAP4-MTBD (to label microtubules) in grey, H2B-mRFP (to label chromosomes) in magenta and EGFP-UtrCH (to label actin) in green are shown.
Movie S4
Super-resolution (Zeiss Airyscan) time lapse movie of spindle actin during anaphase I in a DMSO-treated oocyte. SNAP-MAP4-MTBD (to label microtubules) in grey, H2B-mRFP (to label chromosomes) in magenta and EGFP-UtrCH (to label actin) in green are shown.
Movie S5
Super-resolution (Zeiss Airyscan) time lapse movie of spindle actin during anaphase I in a Cytochalasin D-treated oocyte. SNAP-MAP4-MTBD (to label microtubules) in grey, H2B-mRFP (to label chromosomes) in magenta and EGFP-UtrCH (to label actin) in green are shown.
Movie S6
Time lapse movie of meiotic maturation of a DMSO-treated mouse oocyte expressing EGFP-MAP4-MTBD (to label microtubules) and H2B-mRFP (to label chromosomes)
Movie S7
Time lapse movie of meiotic maturation of a Cytochalasin D-treated mouse oocyte expressing EGFP-MAP4-MTBD (to label microtubules) and H2B-mRFP (to label chromosomes)
Movie S8
Time lapse movie of meiotic maturation of a Fmn2+/+ mouse oocyte expressing EGFP-MAP4-MTBD (to label microtubules) and H2B-mRFP (to label chromosomes)
Movie S9
Time lapse movie of meiotic maturation of Fmn2-/- mouse oocyte expressing EGFP-MAP4-MTBD (to label microtubules) and H2B-mRFP (to label chromosomes)
Movie S10
Super-resolution (Zeiss Airyscan) time lapse movie of actin, microtubules and chromosomes in a meiosis II mouse oocyte expressing EGFP-UtrCH (to label actin), SNAP-MAP4-MTBD (to label microtubules) and H2B-mRFP (to label chromosomes)
Movie S11
Time lapse movie of microtubules (labelled with EGFP-MAP4-MTBD) and chromosomes (labelled with H2B-mRFP) in a mouse oocyte acutely treated with DMSO in meiosis II
Movie S12
Time lapse movie of microtubules (labelled with EGFP-MAP4-MTBD) and chromosomes (labelled with H2B-mRFP) in a mouse oocyte acutely treated with Cytochalasin D in meiosis II
Movie S13
Time lapse movie showing chromosome misalignment and mis-segregation in a mouse oocyte expressing H2B-mRFP (to labels chromosomes) and EGFP-MAP4- UtrCH (to enrich spindle actin).