STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles

Rifka Vlijm, Xue Li, Marko Panic, Diana Rüthnick, Shoji Hata, Frank Herrmannsdörfer, Thomas Kuner, Mike Heilemann, Johann Engelhardt, Stefan W. Hell, and Elmar Schiebel
PNAS February 20, 2018. 201716840; published ahead of print February 20, 2018. https://doi.org/10.1073/pnas.1716840115

Supporting Information

  • Download Supporting Information (PDF)
  • Download Appendix (PDF)
  • Download Dataset_S01 (PDF)
  • Download Movie_S01 (AVI) - Centrosome movement in RPE1 cells. The two centrosomes of an interphase cell are kept together in a dynamic way (representative for ∼95% of the cells). mNeonGreen-CEP68 is shown in green; mRuby2-PACT is shown in red and serves as centrosome marker.
  • Download Movie_S02 (AVI) - Centrosome movement in RPE1 cells, similar to Movie S1. The two centrosomes of an interphase cell are kept together in a dynamic way (representative for ∼95% of the cells). mNeonGreen-CEP68 is shown in green; mRuby2-PACT is shown in red and serves as centrosome marker.
  • Download Movie_S03 (AVI) - Centrosome movement in RPE1 cells, similar to Movies S1 and S2. The two centrosomes of an interphase cell are kept together in a dynamic way (representative for ∼95% of the cells). mNeonGreen-CEP68 is shown in green; mRuby2-PACT is shown in red and serves as centrosome marker.
  • Download Movie_S04 (AVI) - Centrosome movement in RPE1 cells. The separated centrosomes of a cell in interphase are joined and stay together thereafter (representative for ∼5% of the cells). mNeonGreen-CEP68 is shown in green; mRuby2-PACT is shown in red and serves as centrosome marker.
  • Download Movie_S05 (AVI) - Centrosome movement in RPE1 cells, similar to Movie S4. The separated centrosomes of a cell in interphase are joined and stay together thereafter (representative for ∼5% of the cells). mNeonGreen-CEP68 is in green; mRuby2-PACT is shown in red and serves as centrosome marker.
  • Download Movie_S06 (AVI) - Centrosome movement in RPE1 cells, similar to Movies S4 and S5. The separated centrosomes of a cell in interphase are joined and stay together thereafter (representative for ∼5% of the cells). mNeonGreen-CEP68 is shown in green; mRuby2-PACT is shown in red and serves as centrosome marker.
  • Download Movie_S07 (AVI) - A 3D image of a rootletin fiber network imaged by 3D-STORM.
  • Download Movie_S08 (AVI) - A 3D image of a single rootletin fiber imaged by 3D-STORM.
  • Download Movie_S09 (AVI) - A 3D image of a CEP68 fiber network imaged by 3D-STORM.

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