Decomposition of time-dependent fluorescence signals reveals codon-specific kinetics of protein synthesis

doi:10.1093/nar/gky1101

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Nucleic Acids Res. 2018 Dec 14; 46(22): 12186–12187.

Published online 2018 Oct 26. doi: 10.1093/nar/gky1101

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Figure 3.

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Measured fluorescence signatures of the in-vitro synthesis of BOF-fMetPhen peptides (colored lines) and best fit in terms of least squares of the theoretical translation model (black lines). The label ‘no EF-G’ indicates translation of poly(U) mRNA in the absence of EF-G.