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Published online 2019 Jun 17. doi: 10.7554/eLife.46269
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Figure 1—figure supplement 3.

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LC-MS quantification of cohesin subunit stoichiometry.

Quantification of cohesin subunits in SCC1 immunoprecipitates from (A) chromatin or (B) soluble extracts isolated from G1, G2 or prometaphase synchronised HeLa cells. Numbers are normalised relative to SCC1 abundance. Data are plotted as mean ± standard deviation of two biological replicates and two technical replicates. For absolute values and exceptions, see Appendix 1—table 2. For individual peptide counts, see Figure 1—figure supplement 4.

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