Science Advances

Supplementary Materials

This PDF file includes:

  • Table S1. Rate constants (s−1) of the key translocation steps with frameshifting EF-G mutants.
  • Table S2. Rate constants of elemental translocation rates for a five-step model A + EF-G = B→C→D→E→F.
  • Fig. S1. Analysis of 0- and −1-frame products of translation.
  • Fig. S2. Characterization of EF-G mutants.
  • Fig. S3. Activity of EF-G mutants in translocation and 0-frame maintenance.
  • Fig. S4. Schematics of the observables used to measure translocation kinetics.
  • Fig. S5. Examples of global fitting results with wt EF-G (4 μM).
  • Fig. S6. Examples of global fitting results with EF-G(Q507D) (4 μM).
  • Fig. S7. Examples of global fitting results with EF-G(H583K) (4 μM).
  • Fig. S8. Examples of global fitting results with EF-G(H583A) (4 μM).
  • Fig. S9. Comparison of the IFIs for wt EF-G (black) and EF-G mutants Q507D (red), H583K (blue), and H583A (green).

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