date: 2021-11-11T12:24:43Z
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pdf:docinfo:title: Novel Biodegradable Composite of Calcium Phosphate Cement and the Collagen I Mimetic P-15 for Pedicle Screw Augmentation in Osteoporotic Bone
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subject: Osteoporotic vertebral fractures often necessitate fusion surgery, with high rates of implant failure. We present a novel bioactive composite of calcium phosphate cement (CPC) and the collagen I mimetic P-15 for pedicle screw augmentation in osteoporotic bone. Methods involved expression analysis of osteogenesis-related genes during osteoblastic differentiation by RT-PCR and immunostaining of osteopontin and Ca2+ deposits. Untreated and decalcified sheep vertebrae were utilized for linear pullout testing of pedicle screws. Bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DEXA). Expression of ALPI II (p < 0.0001), osteopontin (p < 0.0001), RUNX2 (p < 0.0001), and osteocalcin (p < 0.0001) was upregulated after co-culture of MSC with CPC-P-15. BMD was decreased by 28.75%  2.6%. Pullout loads in untreated vertebrae were 1405  6 N (p < 0.001) without augmentation, 2010  168 N (p < 0.0001) after augmentation with CPC-P-15, and 2112  98 N (p < 0.0001) with PMMA. In decalcified vertebrae, pullout loads were 828  66 N (p < 0.0001) without augmentation, 1324  712 N (p = 0.04) with PMMA, and 1252  131 N (p < 0.0078) with CPC-P-15. CPC-P-15 induces osteoblastic differentiation of human MES and improves pullout resistance of pedicle screws in osteoporotic and non-osteoporotic bone.
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dc:title: Novel Biodegradable Composite of Calcium Phosphate Cement and the Collagen I Mimetic P-15 for Pedicle Screw Augmentation in Osteoporotic Bone
modified: 2021-11-11T12:24:43Z
cp:subject: Osteoporotic vertebral fractures often necessitate fusion surgery, with high rates of implant failure. We present a novel bioactive composite of calcium phosphate cement (CPC) and the collagen I mimetic P-15 for pedicle screw augmentation in osteoporotic bone. Methods involved expression analysis of osteogenesis-related genes during osteoblastic differentiation by RT-PCR and immunostaining of osteopontin and Ca2+ deposits. Untreated and decalcified sheep vertebrae were utilized for linear pullout testing of pedicle screws. Bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DEXA). Expression of ALPI II (p < 0.0001), osteopontin (p < 0.0001), RUNX2 (p < 0.0001), and osteocalcin (p < 0.0001) was upregulated after co-culture of MSC with CPC-P-15. BMD was decreased by 28.75%  2.6%. Pullout loads in untreated vertebrae were 1405  6 N (p < 0.001) without augmentation, 2010  168 N (p < 0.0001) after augmentation with CPC-P-15, and 2112  98 N (p < 0.0001) with PMMA. In decalcified vertebrae, pullout loads were 828  66 N (p < 0.0001) without augmentation, 1324  712 N (p = 0.04) with PMMA, and 1252  131 N (p < 0.0078) with CPC-P-15. CPC-P-15 induces osteoblastic differentiation of human MES and improves pullout resistance of pedicle screws in osteoporotic and non-osteoporotic bone.
pdf:docinfo:subject: Osteoporotic vertebral fractures often necessitate fusion surgery, with high rates of implant failure. We present a novel bioactive composite of calcium phosphate cement (CPC) and the collagen I mimetic P-15 for pedicle screw augmentation in osteoporotic bone. Methods involved expression analysis of osteogenesis-related genes during osteoblastic differentiation by RT-PCR and immunostaining of osteopontin and Ca2+ deposits. Untreated and decalcified sheep vertebrae were utilized for linear pullout testing of pedicle screws. Bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DEXA). Expression of ALPI II (p < 0.0001), osteopontin (p < 0.0001), RUNX2 (p < 0.0001), and osteocalcin (p < 0.0001) was upregulated after co-culture of MSC with CPC-P-15. BMD was decreased by 28.75%  2.6%. Pullout loads in untreated vertebrae were 1405  6 N (p < 0.001) without augmentation, 2010  168 N (p < 0.0001) after augmentation with CPC-P-15, and 2112  98 N (p < 0.0001) with PMMA. In decalcified vertebrae, pullout loads were 828  66 N (p < 0.0001) without augmentation, 1324  712 N (p = 0.04) with PMMA, and 1252  131 N (p < 0.0078) with CPC-P-15. CPC-P-15 induces osteoblastic differentiation of human MES and improves pullout resistance of pedicle screws in osteoporotic and non-osteoporotic bone.
pdf:docinfo:creator: Harald Krenzlin, Andrea Foelger, Volker Mailänder, Christopher Blase, Marc Brockmann, Christoph Düber, Florian Ringel and Naureen Keric
meta:author: Harald Krenzlin
meta:creation-date: 2021-10-20T03:14:18Z
created: 2021-10-20T03:14:18Z
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Author: Harald Krenzlin
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dc:description: Osteoporotic vertebral fractures often necessitate fusion surgery, with high rates of implant failure. We present a novel bioactive composite of calcium phosphate cement (CPC) and the collagen I mimetic P-15 for pedicle screw augmentation in osteoporotic bone. Methods involved expression analysis of osteogenesis-related genes during osteoblastic differentiation by RT-PCR and immunostaining of osteopontin and Ca2+ deposits. Untreated and decalcified sheep vertebrae were utilized for linear pullout testing of pedicle screws. Bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DEXA). Expression of ALPI II (p < 0.0001), osteopontin (p < 0.0001), RUNX2 (p < 0.0001), and osteocalcin (p < 0.0001) was upregulated after co-culture of MSC with CPC-P-15. BMD was decreased by 28.75%  2.6%. Pullout loads in untreated vertebrae were 1405  6 N (p < 0.001) without augmentation, 2010  168 N (p < 0.0001) after augmentation with CPC-P-15, and 2112  98 N (p < 0.0001) with PMMA. In decalcified vertebrae, pullout loads were 828  66 N (p < 0.0001) without augmentation, 1324  712 N (p = 0.04) with PMMA, and 1252  131 N (p < 0.0078) with CPC-P-15. CPC-P-15 induces osteoblastic differentiation of human MES and improves pullout resistance of pedicle screws in osteoporotic and non-osteoporotic bone.
Keywords: osteoporosis; osteoporotic vertebral fractures; polymethylmethacrylate; calcium phosphate cement; collagen I mimetic P-15
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dc:creator: Harald Krenzlin
description: Osteoporotic vertebral fractures often necessitate fusion surgery, with high rates of implant failure. We present a novel bioactive composite of calcium phosphate cement (CPC) and the collagen I mimetic P-15 for pedicle screw augmentation in osteoporotic bone. Methods involved expression analysis of osteogenesis-related genes during osteoblastic differentiation by RT-PCR and immunostaining of osteopontin and Ca2+ deposits. Untreated and decalcified sheep vertebrae were utilized for linear pullout testing of pedicle screws. Bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DEXA). Expression of ALPI II (p < 0.0001), osteopontin (p < 0.0001), RUNX2 (p < 0.0001), and osteocalcin (p < 0.0001) was upregulated after co-culture of MSC with CPC-P-15. BMD was decreased by 28.75%  2.6%. Pullout loads in untreated vertebrae were 1405  6 N (p < 0.001) without augmentation, 2010  168 N (p < 0.0001) after augmentation with CPC-P-15, and 2112  98 N (p < 0.0001) with PMMA. In decalcified vertebrae, pullout loads were 828  66 N (p < 0.0001) without augmentation, 1324  712 N (p = 0.04) with PMMA, and 1252  131 N (p < 0.0078) with CPC-P-15. CPC-P-15 induces osteoblastic differentiation of human MES and improves pullout resistance of pedicle screws in osteoporotic and non-osteoporotic bone.
dcterms:created: 2021-10-20T03:14:18Z
Last-Modified: 2021-11-11T12:24:43Z
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title: Novel Biodegradable Composite of Calcium Phosphate Cement and the Collagen I Mimetic P-15 for Pedicle Screw Augmentation in Osteoporotic Bone
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pdf:docinfo:keywords: osteoporosis; osteoporotic vertebral fractures; polymethylmethacrylate; calcium phosphate cement; collagen I mimetic P-15
pdf:docinfo:modified: 2021-11-11T12:24:43Z
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creator: Harald Krenzlin
dc:subject: osteoporosis; osteoporotic vertebral fractures; polymethylmethacrylate; calcium phosphate cement; collagen I mimetic P-15
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