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. 2023 Jan 20.
doi: 10.1101/pdb.prot107750. Online ahead of print.

Sequencing the Immunoglobulin Heavy-Chain Locus (IgH) in Turquoise Killifish ( Nothobranchius furzeri)

Jens Seidel  1 , William John Bradshaw  1 , Dario Riccardo Valenzano  2
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Sequencing the Immunoglobulin Heavy-Chain Locus (IgH) in Turquoise Killifish ( Nothobranchius furzeri)

Jens Seidel et al. Cold Spring Harb Protoc. .

Abstract

B cells undergo a process of somatic recombination leading to the synthesis of a staggering diversity of transmembrane and secreted antibodies. This process dates back to the evolution of jawed vertebrates and is pivotal to the origin of lymphocyte-based adaptive immune responses. Here, we optimized a sequencing-based protocol to characterize B-cell-specific IgH transcripts from bulk RNA in the African turquoise killifish, an emerging model organism characterized by naturally short life span and by a vast range of age-dependent dysfunctions.

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