Spheroid formation of human thyroid cancer cells in an automated culturing 
system during the Shenzhou-8 Space mission

Pietsch, Jessica; Ma, Xiao; Wehland, Markus; Aleshcheva, Ganna; Schwarzwaelder, Achim; Segerer, Jurgen; Birlem, Maria; Horn, Astrid; Bauer, Johann; Infanger, Manfred; Grimm, Daniela

doi:10.1016/j.biomaterials.2013.06.054

Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission

Pietsch, J., Ma, X., Wehland, M., Aleshcheva, G., Schwarzwaelder, A., Segerer, J., Birlem, M., Horn, A., Bauer, J., Infanger, M., & Grimm, D. (2013). Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission. BIOMATERIALS, 34(31), 7694-7705. doi:10.1016/j.biomaterials.2013.06.054.

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アイテムのパーマリンク: https://hdl.handle.net/11858/00-001M-0000-0014-5BE3-7 版のパーマリンク: https://hdl.handle.net/11858/00-001M-0000-0014-5BE4-5

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Pietsch, Jessica¹, 著者
Ma, Xiao¹, 著者
Wehland, Markus¹, 著者
Aleshcheva, Ganna¹, 著者
Schwarzwaelder, Achim¹, 著者
Segerer, Jurgen¹, 著者
Birlem, Maria¹, 著者
Horn, Astrid¹, 著者
Bauer, Johann², 著者
Infanger, Manfred¹, 著者
Grimm, Daniela¹, 著者

所属:
1external, ou_persistent22
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170

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キーワード: RANDOM POSITIONING MACHINE; HUMAN ENDOTHELIAL-CELLS; TISSUE GROWTH-FACTOR; SIMULATED MICROGRAVITY; CARCINOMA CELLS; EXTRACELLULAR-MATRIX; VASCULOGENIC MIMICRY; PARABOLIC FLIGHT; ALTERED GRAVITY; IN-VITROBiocompatibility; Bioreactor; Growth factors; Apoptosis; Polyetheretherketone; Thyroid cancer;

要旨: Human follicular thyroid cancer cells were cultured in Space to investigate the impact of microgravity on 3D growth. For this purpose, we designed and constructed a cell container that can endure enhanced physical forces, is connected to fluid storage chambers, performs media changes and cell harvesting automatically and supports cell viability. The container consists of a cell suspension chamber, two reserve tanks for medium and fixative and a pump for fluid exchange. The selected materials proved durable, non-cytotoxic, and did not inactivate RNAlater. This container was operated automatically during the unmanned Shenzhou-8 Space mission. FTC-133 human follicular thyroid cancer cells were cultured in Space for 10 days. Culture medium was exchanged after 5 days in Space and the cells were fixed after 10 days. The experiment revealed a scaffold-free formation of extraordinary large three-dimensional aggregates by thyroid cancer cells with altered expression of EGF and CTGF genes under real microgravity. (C) 2013 Elsevier Ltd. All rights reserved.

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言語: eng - English

日付: 出版: 2013-10

出版の状態: 出版

ページ: 12

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査読: 査読あり

識別子（DOI, ISBNなど）: ISI: 000323459100014
DOI: 10.1016/j.biomaterials.2013.06.054

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出版物名: BIOMATERIALS

種別: 学術雑誌

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出版社, 出版地: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND : ELSEVIER SCI LTD

ページ: - 巻号: 34 (31) 通巻号: - 開始・終了ページ: 7694 - 7705 識別子（ISBN, ISSN, DOIなど）: ISSN: 0142-9612

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