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  The Deoxyglucose Method for Insects: towards Electron Microscopical Resolution

Buchner, E., & Buchner, S. (1981). The Deoxyglucose Method for Insects: towards Electron Microscopical Resolution. European Neurology, 20(3), 152-156.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-E8D0-A Version Permalink: https://hdl.handle.net/21.11116/0000-0005-E8D1-9
Genre: Conference Paper

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https://www.karger.com/Article/Abstract/115224 (Publisher version)
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 Creators:
Buchner, E1, 2, Author           
Buchner, S1, 2, Author           
Affiliations:
1Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497802              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              

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 Abstract: The problems of extending the deoxyglucose method for insects to electron microscopical resolution are discussed. It is calculated that several curies of 3H-deoxyglucose per kilogram body weight may be needed for identification of activity in ultra-thin sections. Oral application may be superior to injection. Incorporation of experimental and control stimulus in the same animal seems appropriate. Freeze-drying may turn out to be the best method of preparing the tissue for plastic embedding. The problem of cutting on a water surface is discussed. It is demonstrated that freeze-drying of Drosophila results in structural preservation sufficient for cell identification in the cervical connective. Autoradiography of ultra-thin sections has not yet been carried out.

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 Dates: 1981-05
 Publication Status: Issued
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1159/000115224
 Degree: -

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Title: International Congress on Deoxyglucose Uptake and Oxygen Consumption: a Metabolic Approach to Cerebral Function 1980
Place of Event: Paris, France
Start-/End Date: 1980-09-19 - 1980-09-20

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Title: European Neurology
  Other : Eur. Neurol.
Source Genre: Journal
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Publ. Info: Basel : S. Karger.
Pages: - Volume / Issue: 20 (3) Sequence Number: - Start / End Page: 152 - 156 Identifier: ISSN: 0014-3022
CoNE: https://pure.mpg.de/cone/journals/resource/954925398489