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  Stability of BDNF in human samples stored up to 6 months and correlations of serum and EDTA-plasma concentrations

Polyakova, M., Schlögl, H., Sacher, J., Schmidt-Kassow, M., Kaiser, J., Stumvoll, M., et al. (2017). Stability of BDNF in human samples stored up to 6 months and correlations of serum and EDTA-plasma concentrations. International Journal of Molecular Sciences, 18(6): 1189. doi:10.3390/ijms18061189.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-E166-D Version Permalink: https://hdl.handle.net/21.11116/0000-0004-4D84-1
Genre: Journal Article

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 Creators:
Polyakova, Maryna1, 2, Author           
Schlögl , Haiko 2, Author
Sacher, Julia1, 2, Author           
Schmidt-Kassow , Maren 2, Author
Kaiser, Jochen 2, Author
Stumvoll, Michael 2, Author
Kratzsch, Jürgen 2, Author
Schroeter, Matthias L.1, 2, Author           
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1Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634549              
2External Organizations, ou_persistent22              

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Free keywords: BDNF; brain-derived neurotrophic factor; serum; EDTA-plasma; stability; inter-assay precision; acceptable change limit
 Abstract: Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at −80 °C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations

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Language(s): eng - English
 Dates: 2017-03-302017-05-302017-06
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/ijms18061189
PMID: 28587193
PMC: PMC5486012
 Degree: -

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Project name : German Consortium for Frontotemporal Lobar Degeneration
Grant ID : O1GI1007A
Funding program : -
Funding organization : German Federal Ministry of Education and Research (BMBF)
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Funding organization : LIFE – Leipzig Research Center for Civilization Diseases at the University of Leipzig
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Funding organization : European Union (EU)
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Funding program : European Regional Development Fund
Funding organization : European Commission (EC)
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Funding organization : Free State of Saxony
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Grant ID : PDF-IRG-1307
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Funding organization : Parkinson’s Disease Foundation
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Grant ID : MJFF-11362
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Funding organization : Michael J. Fox Foundation
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Funding organization : International Max Planck Research School on Neuroscience of Communication: Function, Structure, and Plasticity (IMPRS NeuroCom)
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Grant ID : SCHM 2693/1-2
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Funding organization : German Research Foundation (DFG)
Project name : Obesity Mechanisms / SFB 1052
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Funding organization : German Research Foundation (DFG)
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Funding organization : Max Planck Society

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Title: International Journal of Molecular Sciences
  Abbreviation : Int. J. Mol. Sci.
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 18 (6) Sequence Number: 1189 Start / End Page: - Identifier: ISSN: 1422-0067
CoNE: https://pure.mpg.de/cone/journals/resource/1422-0067