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  Identification of spathulenol in Salvia mirzayanii and the immunomodulatory effects

Ziaei, A., Ramezani, M., Wright, L., Paetz, C., Schneider, B., & Amirghofran, Z. (2011). Identification of spathulenol in Salvia mirzayanii and the immunomodulatory effects. Phytotherapy Research, 25(4), 557-562. doi:10.1002/ptr.3289.

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NMR153.pdf (Publisher version), 494KB
 
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Ziaei, Akram, Author
Ramezani, Mohammad, Author
Wright, Louwrance1, Author           
Paetz, Christian2, Author           
Schneider, Bernd2, Author           
Amirghofran, Zahra, Author
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
2Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society, ou_421898              

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 Abstract: The methanol extract of Salvia mirzayanii has shown an immunomodulatory effect on peripheral blood lymphocytes. Bioassay‐guided fractionation using a lymphocyte proliferation assay on Salvia mirzayanii was performed in order to purify and identify the active compounds. Fractionation of the methanol extract and purification of the components using normal column chromatography and preparative thin layer chromatography resulted in identification of the bioactive compound, spathulenol, with an immunoinhibitory effect. Identification of this compound was performed by 1D and 2D NMR methods and HRMS. Treatment of activated lymphocytes with a concentrated fraction containing 62% of spathulenol (SP) showed a decrease in the proliferation of lymphocytes with an IC50 of 85.4 ± 11.08 µg/mL. Flow cytometry analysis using annexin V and propidium iodide staining of the stimulated peripheral blood lymphocytes in the presence of SP demonstrated a dose dependent increase in the percentage of apoptotic cells (IC50; 77.2 ± 5.31 µg/mL). No significant increase in caspase 3 activity in a 20 h treatment of stimulated lymphocytes compared with the control was observed. In conclusion, this study identified the possible activity of spathulenol as one of the immunomodulatory compounds present in Salvia mirzayanii. SP showed the capacity to inhibit proliferation in the lymphocytes and to induce apoptosis in these cells possibly through a caspase‐3 independent pathway.

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 Dates: 20102011
 Publication Status: Published in print
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 Identifiers: Other: NMR153
DOI: 10.1002/ptr.3289
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Title: Phytotherapy Research
  Other : Phytotherapy research [Elektronische Ressource] : an international journal devoted to pharmacological and toxicological evaluation of natural product derivatives
  Abbreviation : Phytother. Res.
Source Genre: Journal
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Publ. Info: Bognor Regis : Wiley
Pages: - Volume / Issue: 25 (4) Sequence Number: - Start / End Page: 557 - 562 Identifier: ISSN: 1099-1573
CoNE: https://pure.mpg.de/cone/journals/resource/1099-1573