English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Acetylcholine, GABA and glutamate induce ionic currents in cultured antennal lobe neurons of the honeybee, Apis mellifera.

Barbara, G. S., Zube, C., Rybak, J., Gauthier, M., & Grünewald, B. (2005). Acetylcholine, GABA and glutamate induce ionic currents in cultured antennal lobe neurons of the honeybee, Apis mellifera. Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology, 191(9), 823-836. doi:10.1007/s00359-005-0007-3.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-0F6A-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-C58E-6
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Barbara, Guillaume Stephane1, Author
Zube, Christina2, Author              
Rybak, Jürgen3, Author              
Gauthier, Monique1, Author
Grünewald, Bernd3, Author
Affiliations:
1Centre de Recherche sur la Cognition Animale- CNRS - Université Paul Sabatier , 118 rte de Narbonne, 31062 Toulouse, France, ou_persistent22              
2Department of Behavioral Physiology and Sociobiology, Universität Würzburg, Biozentrum, am Hubland, 97074 Würzburg, ou_persistent22              
3Institut für Biologie, Freie Universität Berlin, Königin-Luise-Straße 28-30, 14195 Berlin, ou_persistent22              

Content

show
hide
Free keywords: Insect, Neurotransmitter, Acetylcholine receptor, Patch Clamp, Glutamate Receptor
 Abstract: The honeybee, Apis mellifera, is a valuable model system for the study of olfactory coding and its learning and memory capabilities. In order to understand the synaptic organisation of olfactory information processing, the transmitter receptors of the antennal lobe need to be characterized. Using whole-cell patch-clamp recordings, we analysed the ligand-gated ionic currents of antennal lobe neurons in primary cell culture. Pressure applications of acetylcholine (ACh), gamma-amino butyric acid (GABA) or glutamate induced rapidly activating ionic currents. The ACh-induced current flows through a cation-selective ionotropic receptor with a nicotinic profile. The ACh-induced current is partially blocked by alpha-bungarotoxin. Epibatidine and imidacloprid are partial agonists. Our data indicate the existence of an ionotropic GABA receptor which is permeable to chloride ions and sensitive to picrotoxin (PTX) and the insecticide fipronil. We also identified the existence of a chloride current activated by pressure applications of glutamate. The glutamate-induced current is sensitive to PTX. Thus, within the honeybee antennal lobe, an excitatory cholinergic transmitter system and two inhibitory networks that use GABA or glutamate as their neurotransmitter were identified.

Details

show
hide
Language(s):
 Dates: 2005-07-26
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1007/s00359-005-0007-3
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
  Other : J. Comp. Physiol. A -Neuroethol. Sens. Neural Behav. Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 191 (9) Sequence Number: - Start / End Page: 823 - 836 Identifier: ISSN: 0340-7594
CoNE: https://pure.mpg.de/cone/journals/resource/954925519626