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  Involvement of an NAD(P)H oxidase in the elicitor-inducible oxidative burst of soybean

Mithöfer, A., Daxberger, A., FromholdTreu, D., & Ebel, J. (1997). Involvement of an NAD(P)H oxidase in the elicitor-inducible oxidative burst of soybean. Phytochemistry, 45(6), 1101-1107. doi:10.1016/S0031-9422(97)00133-7.

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Mithöfer, Axel1, Author           
Daxberger, A., Author
FromholdTreu, D., Author
Ebel, J., Author
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1External Organizations, ou_persistent22              

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Free keywords: glycine max fabaceae beta-glucan elicitor oxidative burst nad(p)h oxidase glyceollins high-affinity binding host-pathogen interactions hypersensitive disease resistance glucoside phytoalexin elicitor hyphal wall components cultured plant-cells active oxygen glycine-max phytophthora-infestans peroxidase-activities
 Abstract: The production of H2O2 in soybean (Glycine max L. cv Harosoy 63) suspension cells upon treatment with a 1,3-1,6-beta-glucan elicitor fraction from the cell walls of Phyrophthora sojae was investigated. The beta-glucan elicitor used in these studies was specifically recognized by the soybean beta-glucan-binding proteins and induced phytoalexin synthesis in the cell culture. Production of H2O2 was measured by the in situ oxidation of o-dianisidine and was both a time- and a concentration-dependent process, with a maximal response occurring after 20-25 min. Inhibitor studies showed that catalase, peroxidase inhibitors and ascorbate abolished the H2O2-mediated o-dianisidine oxidation. Diphenyleneiodonium, but not p-hydroxymercury benzoic acid, both known inhibitors of the mammalian NADPH oxidase involved in the oxidative burst of phagocytes, was a strong inhibitor of the inducible H2O2 production with a concentration for 50% inhibition of 4 mu M. The inhibitor studies also indicated that the oxidase might be at least in part responsible for H2O2 production by the cells. Furthermore, diphenyleneiodonium was the only inhibitor of O-2(-) synthesis in cell-free analyses with a concentration for 50% inhibition of 2.2 mu M. Neither ascorbate nor diphenyleneiodonium were able to inhibit elicitor-induced phytoalexin accumulation, indicating that in soybean H2O2 and O-2(-) may not be directly responsible for phytoalexin production, but may be an independent defence response. (C) 1997 Elsevier Science Ltd. All rights reserved.

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 Dates: 1997
 Publication Status: Issued
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 Identifiers: Other: EXT052
DOI: 10.1016/S0031-9422(97)00133-7
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Title: Phytochemistry
  Other : Phytochem
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 45 (6) Sequence Number: - Start / End Page: 1101 - 1107 Identifier: ISSN: 0031-9422
CoNE: https://pure.mpg.de/cone/journals/resource/954925433416