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Carbon; Electron microscopy; Specimen preparation; Sample preparation [A0660E]; Electron and ion microscopes and techniques [A0780]; Thin film growth, structure, and epitaxy [A6855]
Abstract:
A relatively simple modification has been designed and built for a commercial freeze-fracture machine which makes it possible to evaporate carbon onto frozen-hydrated specimens after the specimen grid has been mounted into a side-entry electron microscope cryo-holder. The evaporated carbon film greatly improves the stability of self-supported, vitreous ice films during exposure to the electron beam. Specimen charging is also greatly reduced. As a consequence, the image resolution attainable with carbon-coated, vitreous ice specimens appears to be limited only by the mechanical stability of the cryostage itself. (13 References).
